Class B CpG oligonucleotide - Human & mouse TLR9 ligand
CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs). These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. CpG ODNs are recognized by Toll-like receptor 9 (TLR9) leading to strong immunostimulatory effects.
ODN BW006 is a type B CpG ODN containing twice the optimal motif in human, GTCGTT. B-class CpG ODNs contain a full phosphorothioate backbone with one or more CpG dinucleotides. They strongly activate B cells but weakly stimulate IFN-α secretion. ODN BW006 promotes strong Th1 responses.
ODN BW007 is a control ODN that contains GpC dinucleotides instead of CpGs and can be used as a negative control together with ODN BW006.