MDP Rhodamine [ Discontinued ]
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Rhodamine-labeled NOD2 Agonist
Muramyl dipeptide (MDP) is the minimal bioactive peptidoglycan motif common to all bacteria, the essential structure required for adjuvant activity in vaccines.
MDP has been shown to be recognized by NOD2, but not TLR2, nor TLR2/1 or TLR2/6 associations [1,2]. This recognition is highly stereospecific of the L-D isomer, excluding any reaction to the D-D or L-L analogs2.
The potent adjuvant activity of MDP may also be linked to an activation of the CIAS1/NALP3/Cryopyrin inflammasome .
MDP Rhodamine is coupled via a 6-aminohexanoic acid spacer molecule at the C6 position of the muric acid. This spacer linker arm minimizes potential steric hindrance effects.
1. Girardin SE. et al., 2003. Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection. J Biol Chem. 278(11):8869-72.
2. Inohara N. et al., 2003. Host recognition of bacterial muramyl dipeptide mediated through NOD2. Implications for Crohn's disease.J Biol Chem. 278(8):5509-12.
3. Martinon F. et al., 2004. Identification of bacterial muramyl dipeptide as activator of the NALP3/cryopyrin inflammasome. Curr Biol. 14(21):1929-34.
Specificity: NOD2 agonist
Working concentration: 1 - 10 µg/ml
Endotoxin level: EndoFit™
Solubility: DMSO (10 mg/ml)
Molecular weight: 1141.72
Spectral Properties of Rhodamine
- Excitation λ max: 552 nm
- Emission λ max: 570 nm
MDP Rhodamine is provided as a lyophilized powder:
- 500 μg Muramyl dipeptide (MDP) labeled with Rhodamine
- 1.5 ml sterile endotoxin-free water
MDP Rhodamine is shipped at room temperature.
Store at -20°C. Protect from light. Upon resuspension, aliquote MDP Rhodamine and store at -20°C.
Product is stable 6 months at -20°C when properly stored.
Avoid repeated freeze-thaw cycles.Back to the top