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MAb-mTLR9

MAb-mTLR9 Unit size Cat. code Docs Qty Price
Mouse TLR9 Detection antibody - Monoclonal Mouse IgG2a (5G5)
100 µg
mab-mtlr9
+-
$325.00

Mouse TLR9 Detection antibody - Monoclonal Mouse IgG2a (5G5)

MAb mTLR9 (5G5) is a monoclonal antibody that reacts with mouse Toll-like receptor 9 (TLR9, CD289). MAb mTLR9 is weakly cross reactive with human TLR9.

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Specifications

Clonality: Monoclonal antibody

Applications: Flow cytometry, Western blotting and immuno assays as a detection antibody. It can also be used for immunohistology on frozen tissue sections and immunoprecipitation.

Specificity: Mouse TLR9 (weak human TLR9 activity)

Clone: 5G5

Isotype: Mouse IgG2a

Formulation: PBS pH 7.4, 0.05% sodium azide

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Contents

  • 100 μg purified monoclonal anti-mTLR9 antibody (MAb-mTLR9) provided lyophilized.

room temperature Product is shipped at room temperature.

store Store lyophilized MAb-mTLR9 at -20̊C.

stability Product is stable for 1 year. Resuspended MAb-mTLR9 is stable up to 1 year when stored at -20̊C

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Description

Toll-like receptors (TLRs) play a critical role in early innate immunity to invading pathogens by sensing microorganisms. These evolutionary conserved receptors recognize highly conserved structural motifs only expressed by microbial pathogens, called pathogen-associated microbial patterns (PAMPs). Stimulation of TLRs by PAMPs initiates a signaling cascade leading to the secreti-on of proinflammatory cytokines following NF-κB activation. To date ten human and twelve murine TLRs have been characterized, TLR1 to TLR10 in humans, and TLR1 to TLR9, TLR11, TLR12 (aka TLR11) and TLR13 in mice, the homolog of TLR10 being a pseudogene. TLR9 is expressed in the ER of resting cells in contrast to most TLRs that are located on the plasma membrane [1].

TLR9 recognizes specific unmethylated CpG-ODN sequences, that distinguish bacerial DNA from mammalian DNA. TLR9 engages an intracellular pathway that involves MyD88 leading to NF-κB translocation [2]. TLR9 recognizes different CpG motifs; the optimal sequences being GTCGTT and GACGTT for hTLR9 and mTLR9 respectively [3]. TLR9 binds directly to CpG DNA and recognizes specifically CpG DNA that is unmethylated and single stranded [4].

 

1. Latz E. et al., 2004. TLR9 signals after translocating from the ER to CpG DNA in the lysosome. Nat Immunol. 5(2):190-8.
2. Chuang TH. et al., 2002. Toll-like receptor 9 mediatese CpG-DNA signlaing. J Leukoc Biol, 71(3):538-44.
3. Bauer S. et al., 2001. Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. Proc Natl. Acad Sci USA, 98(16):9237-42.
4. Rutz M. et al., 2004. Toll-like receptor 9 binds single-stranded CpG-DNA in a sequence- and pH-dependent manner. Eur J Immunol. 34(9):2541-50.

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