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MAb-mTLR4/MD2 [ Discontinued ]

MAb-mTLR4/MD2 Unit size Cat. code Docs Qty Price
Mouse TLR4/MD2 Detection antibody
100 µg
mab-mtlr4md2
+-
$315.00
discontinued

DISCONTINUED

MAb mTLR4/MD2 monoclonal antibody reacts with mouse Toll-like receptor 4 (TLR4, CD284) that is associated with MD2. 

MAb mTLR4/MD2 was shown to coprecipitate MD2 (30 kDa) with TLR4 (100 kDa).

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Specifications

Clonality: Monoclonal antibody MTS510

Applications: Flow cytometry, immunoprecipitation, immunohistochemical staining of frozen tissue sections and neutralization (antagonist of TLR4)

Specificity: Mouse TLR4/MD2

Clone: MTS510

Isotype: Rat IgG2a

Formulation: PBS pH 7.4

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Contents

MAb-mTLR4/MD2 provided lyophilized.

  • 100 μg purified monoclonal anti-mTLR4/MD2 antibody

Product is shipped at room temperature.

Store lyophilized MAb-mTLR4/MD2 at -20°C.

Product is stable for 1 year. Resuspended MAb-mTLR4/MD2 is stable up to 1 year when stored at -20°C.

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Description

Toll-like receptors (TLRs) play a critical role in early innate immunity to invading pathogens by sensing microorganisms.

These evolutionary conserved receptors recognize highly conserved structural motifs only expressed by microbial pathogens, called pathogen-associated microbial patterns (PAMPs).

Stimulation of TLRs by PAMPs initiates a signaling cascade leading to the secretion of proinflammatory cytokines following NF-κB activation.

To date ten human and twelve murine TLRs have been characterized, TLR1 to TLR10 in humans, and TLR1 to TLR9, TLR11, TLR12 and TLR13 in mice, the homolog of TLR10 being a pseudogene.

TLR4, the first human TLR identified, is the receptor for Gram-negative lipopolysaccharide (LPS). The TLR4 gene was shown to be mutated in C3H/HeJ and C57BL/10ScCr mice, both of which are low responders to LPS [1].

However, TLR4 alone is not sufficient to confer LPS responsiveness. TLR4 requires MD-2, a secreted molecule, to functionally interact with LPS [2].

Furthermore, a third protein, called CD14, was shown to participate in LPS signaling, leading to NF-κB translocation.

This signaling is mediated through several adaptor proteins: MyD88 TIRAP/Mal [3] , TRIF/TICAM1 and TRAM/TICAM2 [4].

 

1. Poltorak A. et al., 1998. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science, 282(5396):2085-8.
2. Shimazu R. et al., 1999. MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med, 189(11):1777-82.
3. Horng T. GM. Barton, and R. Medzhitov, 2001. TIRAP: an adapter molecule in the Toll signaling pathway. Nat Immunol, 2(9):835-41.
4. Fitzgerald KA. et al., 2003. LPS-TLR4 Signaling to IRF-3/7 and NF-{kappa}B Involves the Toll Adapters TRAM and TRIF. J Exp Med. 198(7):1043-1055.

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