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MAb-hTLR3

MAb-hTLR3 Unit size Cat. code Docs Qty Price
Human TLR3 Detection antibody - Monoclonal Mouse IgG1 (TLR3.7)
100 µg
mab-htlr3
+-
$325.00

Human TLR3 Detection antibody - Monoclonal Mouse IgG1 (TLR3.7)

MAb hTLR3 (TLR3.7) is a monoclonal antibody that reacts with human Toll-like receptor 3 (TLR3, CD283).

MAb hTLR3 was shown to suppress poly(I:C)-mediated IFN-β production by human fibroblasts naturally expressing TLR3 on their surface [1].
 

Reference:

1. Matsumoto M. et al., 2002. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem Biophys Res Commun. 293:1364-9.

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Specifications

Clonality: Monoclonal antibody

Applications: Flow cytometry, Western blot

Specificity: Human TLR3

Clone: TL3.7

Isotype: Mouse IgG1

Formulation: Phosphate buffered saline (PBS; pH 7.4), 0.05% sodium azide

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Contents

  • 100 μg purified monoclonal anti-hTLR3 antibody (MAb-hTLR3) provided lyophilized.

room temperature Product is shipped at room temperature.

store Store lyophilized MAb-hTLR3 at -20°C.

stability Product is stable for 1 year. Resuspended MAb-hTLR3 is stable up to 1 year when stored at -20°C.

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Description

Toll-Like receptors (TLRs) play a critical role in early innate immunity to invading pathogens by sensing microorganisms. These evolutionary conserved receptors recognize highly conserved structural motifs only expressed by microbial pathogens, called pathogen-associated microbial patterns (PAMPs). Stimulation of TLRs by PAMPs initiates a signaling cascade leading to the secretion of proinflammatory cytokines following NF-κB activation. To date ten human and twelve murine TLRs have been characterized, TLR1 to TLR10 in humans, and TLR1 to TLR9, TLR11, TLR12 (aka TLR11) and TLR13 in mice, the homolog of TLR10 being a pseudogene.

TLR3 recognizes double-stranded RNA (dsRNA), a molecular pattern associated with viral infection. Stimulation with poly(I:C), a synthetic analog of dsRNA, was shown to induce hyporesponsiveness in TLR3-deficient mice and considerable responsiveness in HEK293 cells expressing TLR3 [1], suggesting a specific recognition to poly(I:C) by TLR3. TLR3 signals mainly through a MyD88-independent pathway involving the TRIF/TICAM1 adapter protein that leads to the production of IFN-β and causes dendritic cells to mature [2].

 

1. Alexopoulou L. et al., 2001. Recognition of double-stranded RNA and activation of NF- kappaB by Toll-like receptor 3. Nature, 413(6857):732-8.
2. Yamamoto M. et al., 2002. Cutting edge: A novel Toll/Il-1 receptor domain-containing adapter that preferentially activates the IFN-β promoter in the Toll-like receptor signaling. J Immunol, 169(12):6668-6672.

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