LY294002

PI3K inhibitor - Autophagy inhibitor

LY294002 is a potent, cell-permeable inhibitor of phosphatidylinositol 3-kinase (PI3K) that acts on the ATP binding site of the enzyme [1].
The PI3K pathway is extensively studied for its role in inhibiting apoptosis. PI3K is also known to regulate TLR-mediated inflammatory responses [2, 3].
Furthermore, PI3K is required for autophagy [4]. Autophagy is a complex pathway in which cell material can be sequestered and delivered to the lysosome for degradation. Inhibition of PI3K with LY294002 can inhibit autophagic sequestration [4].

References:

1. Vlahos CJ. et al., 1994. A specific inhibitor of phosphatidylinositol 3-kinase, 2-(4- morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002). J. Biol. Chem 69(7):5241-8.
2. Kuo CC. et al., 2006. Class I and III Phosphatidylinositol 3'-Kinase Play Distinct Roles in TLR Signaling Pathway. J. Immunol., 176: 5943 - 5949.
3. Guiducci C. et al., 2008. PI3K is critical for the nuclear translocation of IRF-7 and type I IFN production by human plasmacytoid predendritic cells in response to TLR activation. J. Exp. Med., 205: 315 - 322.
4. Blommaart EF. et al., 1997. The phosphatidylinositol 3-kinase inhibitors wortmannin and LY294002 inhibit autophagy in isolated rat hepatocytes. Eur. J. Biochem. 243: 240–246.

Specifications

Working concentration: 1 -100 μM

Purity: ≥99%

Solubility: DMSO (10 mg/ml)

CAS number: 154447-36-6

Synonym: 2-(4-morpholino)-8-phenyl-4H-1-benzopyran-4-one

Molecular weight: 307.35

Contents

LY294002 is provided as a powder.

• 5 mg LY294002

LY294002 is shipped at room temperature.

Store at -20°C.

Solid product is stable 1 year at -20°C.

Details