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Human IFN-beta bioluminescent ELISA kit
Human interferon beta bioluminescent ELISA kit
LumiKine™ hIFN-β is a bioluminescent ELISA kit designed to quantify the levels of human interferon-beta (hIFN-β) in cell culture supernatant, serum and plasma samples.
This kit uses optimized matched pairs of monoclonal capture and detection antibodies to achieve sensitive and accurate measurement of the cytokine. The detection antibody is labeled with biotin to allow subsequent binding to streptavidin-Lucia, streptavidin conjugated to Lucia luciferase, a secreted reporter enzyme.
Levels of hIFN‑β are determined by measuring the luminescence produced by Lucia luciferase after hydrolysis of its substrate, coelenterazine. This substrate is provided in QUANTI-Luc™, a reagent that allows immediate reading of the luminescence.
Target: natural and recombinant human IFN-β
Standard: CHO-expressed human IFN-β
Format: bioluminescent ELISA in white immuno 96-well plate
Measurement: relative light units (RLUs)
Incubation Time: 4.5 hours
Limit of detection: 32 pg/ml
Specificity: no cross-reactivity with mouse IFN-β, human or mouse IFN-α
Sample size and type: 100 µl of cell culture supernatant, serum and plasma
- The sensitivity and specificity of this kit are validated for each lot.
- Rigorous quality control tests are performed to ensure lot-to-lot reproducibility and performance
For five plates:
- 50 µg lyophilized hIFN-β Capture Antibody
- 5 µg lyophilized hIFN-β Biotinylated Antibody (detection antibody)
- 100 ng lyophilized hIFN-b Standard (CHO-expressed cytokine)
- 2 pouches of QUANTI-Luc™
- 1 vial Streptavidin-Lucia
- 5 white flat-bottom MaxiSorp® 96-well plates with 5 plate sealers
Products are shipped at room temperature.
Store at -20°C.
Lyophilized products are stable for 12 months when properly storedBack to the top
The monoclonal hIFN-β Capture Antibody is coated onto the wells of a white immuno 96-well plate.
Samples and the hIFN-β Standard are pipetted into these wells, followed by the addition of the monoclonal Biotinylated Antibody (detection antibody).
During the first incubation, the hIFN-β antigen binds to the immobilized hIFN-β Capture Antibody on one site, and during the second incubation, to the Biotinylated Antibody on a second site.
After removal of the excess detection antibody, Streptavidin-Lucia is added. This streptavidin conjugate binds to the Biotinylated Antibody.
After a short third incubation and washing to remove all the unbound Lucia luciferase, QUANTI-Luc™, a solution that contains the substrate of Lucia luciferase, is added. No further incubation is required. The bioluminescent signal is emitted instantaneously and measured in Relative Light Units (RLU) by a luminometer. The intensity of this signal is directly proportional to the concentration of hIFN-β present in the samples.Back to the top