Four step bioluminescent ELISA kits
LumiKine™ kits are bioluminescent ELISA kits designed to quantify the levels of cytokines in cell culture supernatant, serum and plasma samples using the classical “sandwich” ELISA method.
LumiKine™ kits include a capture antibody, a biotinylated secondary antibody and a streptavidin-Lucia conjugate for detection. This conjugate consists of a streptavidin peptide fused to Lucia luciferase, a small secreted coelenterazine-utilizing luciferase with strong and stable bioluminescence properties. After addition of QUANTI-Luc™, a solution that contains the Lucia luciferase substrate,a bioluminescent signal is instantaneously emitted. This signal is directly proportional to the concentration of the cytokine produced and quantifiable by a luminometer. The streptavidin-Lucia conjugate can be replaced by the streptavidin conjugate of your choice.
Contents: capture antibody, biotinylated antibody, recombinant cytokine, strepatividin-Lucia, QUANTI-Luc™, white immunoplates