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pNiFty3-Lucia

Signal Transduction Reporter Plasmids

pNiFty3-Lucia is a family of inducible reporter plasmids that allow to monitor the activation of key signaling pathways. They feature the Lucia luciferase gene under the control of a synthetic promoter inducible by one or several defined transcription factors activated by these pathways.
This promoter consists of the IFN-β minimal promoter combined with repeated transcription factor binding sites for nuclear factor (NF)-κB, activator protein 1 (AP-1), interferon regulatory factors (IRFs) and/or nuclear factor of activated T-cell (NFAT).
pNiFty3-Lucia plasmids carry the Zeocin™ resistance gene for selection in mammalian cell lines and bacteria.

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