pNiFty3-Lucia family

Signal Transduction Reporter Plasmids

pNiFty3-Lucia is a family of inducible reporter plasmids that allow to monitor the activation of key signaling pathways.

They feature the Lucia luciferase gene under the control of a synthetic promoter inducible by one or several defined transcription factors activated by these pathways.

This promoter consists of the IFN-β minimal promoter combined with repeated transcription factor binding sites for nuclear factor (NF)-κB, activator protein 1 (AP-1), interferon regulatory factors (IRFs) and/or nuclear factor of activated T-cell (NFAT).

pNiFty3-Lucia plasmids carry the Zeocin™ resistance gene for selection in mammalian cell lines and bacteria.