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LAM-MS

LAM-MS Unit size Cat. code Docs Qty Price
Lipoarabinomannan from M. smegmatis
500 µg
tlrl-lams
+-
$258.00
discontinued

DISCONTINUED

Lipoarabinomannan from M. smegmatis

Lipoarabinomannans (LAM) is a lipoglycan found in mycobacterial cell wall.

LAM from the non-pathogenic Mycobacterium smegmatis is a proinflammatory molecule.

LAM-MS activates macrophages in a TLR2-dependent manner.

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Specifications

Specificity: TLR2 agonist

Working concentration: 100 ng - 10 μg/ml

Endotoxin level: <0.125 EU/mg

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Contents

  • 500 μg lipoarabinomannan from Mycobacterium smegmatis (LAM-MS)
  • 1.5 ml sterile endotoxin-free water

room temperature LAM-MS is shipped at room temperature

store Stored at -20˚C.

Upon resuspension, LAM-MS should be aliquoted and stored at 4°C for short term storage or -20˚C for long storage.

stable Product is stable 1 month at 4°C and 6 months at -20°C when properly stored.

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Description

Lipoarabinomannans (LAM) are lipoglycans restricted to the Mycobacterium genus that act as potent modulators of the host immune response. They are found in the envelope of all mycobacteria species, such as the pathogenic strains M. tuberculosis and M. leprae, the vaccine strain, M. bovis BCG, the opportunistic strains M. avium and M. foruitum, and the non-pathogenic strain M. smegmatis. LAM display different immunomodulatory effects depending on their structure. PILAM, which are phosphoinositol-capped LAM and found in nonpathogenic species (M. smegmatis), are proinflammatory molecules whereas ManLAM, which are mannose-capped LAM and found in pathogenic species (M. tuberculosis), are anti-inflammatory molecules [1]. PILAM activates macrophages in a TLR2-dependent manner that seems to involve other TLRs but not TLR4 [2,3].

 

1. Quesniaux VJ. et al., 2004. Toll-like receptor 2 (TLR2)-dependent-positive and TLR2-independent-negative regulation of proinflammatory cytokines by mycobacterial lipomannans. J Immunol. 172(7):4425-34.
2. Tapping RI & Tobias PS., 2003. Mycobacterial lipoarabinomannan mediates physical interactions between TLR1 and TLR2 to induce signaling. J Endotoxin Res. 9(4):264-8.
3. Dao DN. et al., 2004. Mycobacterium tuberculosis lipomannan induces apoptosis and interleukin-12 production in macrophages. Infect Immun. 72(4):2067-74.

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Citations

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