HKPA

Heat Killed P. aeruginosa

Pseudomonas aeruginosa is a virulent gram-negative pathogen that infects patients through the respiratory tract, in particular patients with cystic fibrosis. Heat-killed Pseudomonas aeruginosa (HKPA) initiates host inflammatory responses through TLR2 and TLR5 but not TLR4 [1,2]. The TLR5-mediated response was shown to be induced by flagellin while LPS appears to play an important role in the TLR2-mediated response.

References:

1. Yoshimura A. et al., 1999. Cutting Edge: Recognition of Gram-Positive Bacterial Cell Wall Components by the Innate Immune System Occurs Via Toll-Like Receptor 2. J. Immunol. 163:1–5.
2. Khan AQ. et al., 2005. Both Innate Immunity and Type 1 Humoral Immunity to Streptococcus pneumoniae Are Mediated by MyD88 but Differ in Their Relative Levels of Dependence on Toll-Like Receptor 2. Infect. Immun. 73: 298 - 307.

Specifications

Specificity: TLR2 agonist

Working concentration: 10⁵-10⁷ cells/ml

Solubility: Insoluble (resuspended HKPA results in a cloudy suspension)

Contents

HKPA is provided lyophilized.

• 10¹⁰ freeze-dried cells of Heat Killed Pseudomonas aeruginosa (HKPA)
• 1.5 ml sterile endotoxin-free water

HKPA is shipped at room temperature.

Store at 4°C.

Upon resuspension, prepare aliquots of HKPA and store at 4°C for short term storage or -20°C for long storage.

Product is stable 1 month at 4°C and 6 months at -20°C when properly stored.