HEK-Dual™ TNF-α Cells
|HEK-Dual TNF-α Cells||Unit size||Cat. code||Docs||Qty||Price|
Human and Murine TNF-a SEAP/Lucia
3-7 x 10e6 cells
HEK-Dual™ TNF-α cells were specifically designed for the monitoring of bioactive TNF-α in biological samples by assessing NF-κB activation.
HEK-Dual™ TNF-α cells were derived from the human embryonic kidney 293 cell line by stable co-transfection of two NF-κB-inducible reporter constructs. Another feature of HEK-Dual™ TNF-α cells is their inability to respond to IL-1β.
As a result, HEK-Dual™ TNF-α cells allow the specific study of TNF-α-induced NF-κB activation by monitoring the activity of either SEAP or a secreted luciferase (Lucia), each reporter system presenting specific advantages.
Antibiotic resistance: Zeocin™
• Detection range for human TNF-α using QUANTI-Luc™: 0.5 ng/ml - 1 µg/ml
• Detection range for human TNF-α using QUANTI-Blue™: 1 ng/ml - 1 µg/ml
These products are covered by a Limited Use License (See Terms and Conditions).Back to the top
- 1 vial of HEK-Dual™ TNF-α Cells (3-7 x 10e6 cells)
- 1 ml of Zeocin™ (100 mg/ml).
- 1 ml of Normocin™ (50 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
- 1 pouch of QUANTI-Luc™ (Lucia luciferase detection medium)
Shipped on dry ice (Europe, USA, Canada)Back to the top
HEK-Dual™ TNF-α cells feature two different secreted reporter genes, the SEAP (secreted embryonic alkaline phosphatase) gene and the Lucia gene which encodes a secreted luciferase.
Both reporter genes are under the control of the same NF-κB-inducible promoter consisting of an IFN-β minimal promoter fused to five copies of the NF-κB consensus transcriptional response element and three copies of the c-Rel binding site.
Another feature of HEK-Dual™ TNF-α cells is their inability to respond to IL-1β due to a mutation in IL-1β signaling pathway.
As a result, HEK-Dual™ TNF-α cells allow the specific study of TNF-α-induced NF-κB activation by monitoring the activity of either SEAP or luciferase, each reporter system presenting specific advantages.