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HEK-Dual™ TNF-α Cells

HEK-Dual TNF-α Cells Unit size Cat. code Docs Qty Price
Human and Murine TNF-a SEAP/Lucia
3-7 x 10e6 cells
hkd-tnfa
+-
$1,182.00

HEK-Dual™ TNF-α cells were specifically designed for the monitoring of bioactive TNF-α in biological samples by assessing NF-κB activation.

HEK-Dual™ TNF-α cells were derived from the human embryonic kidney 293 cell line by stable co-transfection of two NF-κB-inducible reporter constructs. Another feature of HEK-Dual™ TNF-α cells is their inability to respond to IL-1β.
As a result, HEK-Dual™ TNF-α cells allow the specific study of TNF-α-induced NF-κB activation by monitoring the activity of either SEAP or a secreted luciferase (Lucia), each reporter system presenting specific advantages.

Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™, a SEAP detection reagent, and QUANTI-Luc™, a luciferase detection reagent.

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Specifications

Antibiotic resistance: Zeocin™

Growth medium: DMEM medium, 2mM L-glutamine,10% FBS supplemented with 100 µg/ml Normocin and 100
µg/ml Zeocin™.

Guaranteed mycoplasma-free


• Detection range for human TNF-α using QUANTI-Luc™: 0.5 ng/ml - 1 µg/ml
• Detection range for human TNF-α using QUANTI-Blue™: 1 ng/ml - 1 µg/ml

These products are covered by a Limited Use License (See Terms and Conditions).

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Contents

Shipped on dry ice (Europe, USA, Canada)

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Description

Simplified NF-kB signaling pathway induced by TNF-alpha in HEK-Dual TNF-alpha cells.HEK-Dual™ TNF-α cells were derived from the human embryonic kidney  293 cell line by stable co-transfection of two NF-κB-inducible reporter  constructs.

 

HEK-Dual™ TNF-α cells feature two different secreted  reporter genes, the SEAP (secreted embryonic alkaline phosphatase) gene  and the Lucia gene which encodes a secreted luciferase.
Both reporter  genes are under the control of the same NF-κB-inducible promoter  consisting of an IFN-β minimal promoter fused to five copies of the  NF-κB consensus transcriptional response element and three copies of the  c-Rel binding site.

Another feature of HEK-Dual™ TNF-α cells is their inability to respond to IL-1β due to a mutation in IL-1β signaling pathway.
As  a result, HEK-Dual™ TNF-α cells allow the specific study of  TNF-α-induced NF-κB activation by monitoring the activity of either SEAP  or luciferase, each reporter system presenting specific advantages.

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Citations

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