HEK-Dual™ TNF-α Cells

September 30^th 2019 - CONTENTS UPDATE NOTIFICATION
Please note that, for your convenience, these cells are now provided with QUANTI-Blue™ Solution.

HEK-Dual™ TNF-α cells were specifically designed for the monitoring of bioactive TNF-α in biological samples by assessing NF-κB activation.

HEK-Dual™ TNF-α cells were derived from the human embryonic kidney 293 cell line by stable co-transfection of two NF-κB-inducible reporter constructs. Another feature of HEK-Dual™ TNF-α cells is their inability to respond to IL-1β.
As a result, HEK-Dual™ TNF-α cells allow the specific study of TNF-α-induced NF-κB activation by monitoring the activity of either SEAP or a secreted luciferase (Lucia), each reporter system presenting specific advantages.

Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™, a SEAP detection reagent, and QUANTI-Luc™, a luciferase detection reagent.

Specifications

Antibiotic resistance: Zeocin™

Growth medium: DMEM medium, 2mM L-glutamine,10% FBS supplemented with 100 µg/ml Normocin and 100
µg/ml Zeocin™.

Guaranteed mycoplasma-free

• Detection range for human TNF-α using QUANTI-Luc™: 0.5 ng/ml - 1 µg/ml
• Detection range for human TNF-α using QUANTI-Blue™: 1 ng/ml - 1 µg/ml

These products are covered by a Limited Use License (See Terms and Conditions).

Contents

• 1 vial of HEK-Dual™ TNF-α Cells (3-7 x 10e6 cells)
• 1 ml of Zeocin™ (100 mg/ml).
• 1 ml of Normocin™ (50 mg/ml)
• 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
• 1 pouch of QUANTI-Luc™ (Lucia luciferase detection medium)

Shipped on dry ice (Europe, USA, Canada)

Description

HEK-Dual™ TNF-α cells feature two different secreted  reporter genes, the SEAP (secreted embryonic alkaline phosphatase) gene  and the Lucia gene which encodes a secreted luciferase.
Both reporter  genes are under the control of the same NF-κB-inducible promoter  consisting of an IFN-β minimal promoter fused to five copies of the  NF-κB consensus transcriptional response element and three copies of the  c-Rel binding site.

Another feature of HEK-Dual™ TNF-α cells is their inability to respond to IL-1β due to a mutation in IL-1β signaling pathway.
As  a result, HEK-Dual™ TNF-α cells allow the specific study of  TNF-α-induced NF-κB activation by monitoring the activity of either SEAP  or luciferase, each reporter system presenting specific advantages.