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HEK-Dual™ Null

HEK-Dual™ Null (NF/IL8) Unit size Cat. code Docs Qty Price
Double readout TLR cells
3-7 x 10e6 cells
hkd-nullni
+-
$960.00

Dual NF-kB and IL-8 reporter HEK293 cells

HEK-Dual™ Null cells are the parental cell line for the HEK-Dual™ TLR cells. They were obtained by stable transfection with two different reporter genes (SEAP and the Lucia luciferase) inducible by NF-kB/AP-1 and IL-8, respectively, together with the triple knockout of TLR3, TLR5 and TNFR.

HEK-Dual™ Null cells respond to NOD1 agonists such as Tri-DAP, as they endogenously express NOD1. They do not respond to TLR agonists or to the cytokine TNF-α.

HEK-Dual™ Null cells are resistant to Zeocin™ and blasticidin. They should be maintained in growth medium supplemented with Zeocin™.

 

1. Ohta K. et al., 2014. TLR-mediated interleukin-8 production by human submandibular gland epithelial cells. Mol Med Rep. 10(5):2377-82.
2. Roebuck KA. et al., 1999. Regulation of interleukin-8 gene expression. J Interferon Cytokine Res. 19(5):429-38. 

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Specifications

Antibiotic resistance: blasticidinZeocin™.

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™.

Guaranteed mycoplasma-free.

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Contents

Shipped on dry ice

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