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HEK-Dual™ Null

HEK-Dual™ Null (NF/IL8) Unit size Cat. code Docs Qty Price
Double readout TLR cells
3-7 x 10e6 cells
hkd-nullni
+-
$994.00

June 12th 2019 - CONTENTS UPDATE NOTIFICATION
Please note that, for your convenience, these cells are now provided with QUANTI-Blue™ Solution.

Dual NF-kB and IL-8 reporter HEK293 cells

HEK-Dual™ Null cells are the parental cell line for the HEK-Dual™ TLR cells. They were obtained by stable transfection with two different reporter genes (SEAP and the Lucia luciferase) inducible by NF-kB/AP-1 and IL-8, respectively, together with the triple knockout of TLR3, TLR5 and TNFR.

HEK-Dual™ Null cells respond to NOD1 agonists such as Tri-DAP, as they endogenously express NOD1. They do not respond to TLR agonists or to the cytokine TNF-α.

HEK-Dual™ Null cells are resistant to Zeocin™ and blasticidin. They should be maintained in growth medium supplemented with Zeocin™.

 

References:

1. Ohta K. et al., 2014. TLR-mediated interleukin-8 production by human submandibular gland epithelial cells. Mol Med Rep. 10(5):2377-82.
2. Roebuck KA. et al., 1999. Regulation of interleukin-8 gene expression. J Interferon Cytokine Res. 19(5):429-38. 

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Specifications

Antibiotic resistance: blasticidinZeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

 

These cells are covered by a Limited Use License (See Terms and Conditions).

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Contents

  • 1 vial containing 3-7 x 106 cells
  • 1 ml of Zeocin™ (100 mg/ml)
  • 1 ml of Normocin™ (50 mg/ml)
  • 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
  • 1 pouch of QUANTI-Luc™ (Lucia luciferase detection medium)

Shipped on dry ice (Europe, USA & Canada)

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