Cyclosporin A

Calcineurin inhibitor

Cyclopsporin A is a calcineurin inhibitor that exerts its immunosuppressive effects through the down-regulation of NFAT (nuclear factor of activated T cells) transcription factor, thus preventing the transcription of T cell effector cytokines [1].

Moreover, NFAT has been implicated in the downstream signaling of Dectin-1 which is important in the innate immune response against fungal infection [2].

Conversely, it has been demonstrated that inhibition of calcineurin in macrophages enhances NF-κB activation [3] and can trigger TLR signaling by activating both the MyD88-dependent and the MyD88-independent pathways [4].

 

1. Chow CW. et al., 1999. Requirement for transcription factor NFAT in interleukin-2 expression. Mol Cell Biol. 19(3): 2300-07.
2. Goodridge et al. 2007. Dectin-1stimulation by Candida albicans yeast or zymosan triggers NFAT activation in macrophages and dendritic cells. J Immunol. 178( 5): 3107-15.
3. Asai T. et al., 2003. Activation of transcription factors AP-1 and NF-κB in chronic cyclosporine A nephrotoxicity: role in beneficial effects of magnesium supplementation. Transplantation 75(7):1040–4.
4. Kang Y. et al., 2007. Calcineurin negatively regulates TLR-mediated activation pathways. J Immunol 179(7):4598–607.

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Specifications

Working concentration: 50 nM - 1.5 μM

CAS number: 59865-13-3

Endotoxin levels: <0.001 EU/µg

Solubility: DMSO (50 mg/ml)

Molecular weight: 1202.61

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Contents

Cyclosporin A is provided as a solid

• 100 mg Cyclosporin A

Cyclosporin A is shipped at room temperature.

Store at -20°C.

Solid product is stable 1 year at -20°C when properly stored.

Upon resuspension, Cyclosporin A should be aliquoted and stored at -20°C.
Protect from light.
Avoid repeated freeze-thaw cycles.
Resuspended product is stable 3 months at -20°C when properly stored.

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