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CL572

CL572 Unit size Cat. code Docs Qty Price
Human TLR2 & mouse and human TLR7 Ligand
500 µg
tlrl-c572
+-
$142.00

CL572 (S-(2-myristoyloxy ethyl)-(R)-cysteinyl 4-((6-amino-2-(butylamino)-8- hydroxy-9H-purin-9-yl)methyl) aniline) is a 8-hydroxy-adenine compound conjugated to a monoacyl-ethyl-cystein group via a glutamic acid derivative.

Monoacy-ethyl-cystein-containing dipeptides have been recently shown to specifically activate human TLR2 (Agnihotri G. et al., 2011). Indeed, CL572 is a robust inducer of human TLR2 but is unable to stimulate mouse TLR2. CL572 is also a potent inducer of TLR7.

Agnihotri G. et al., 2011. Structure-activity relationships in toll-like receptor 2-agonists leading to simplified monoacyl lipopeptides. J Med Chem. 54(23):8148-60.

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Specifications

Specificity: human TLR2 and TLR7 agonist

Synonym: S-(2-myristoyloxy ethyl)- (R)-cysteinyl 4- ((6-amino-2- (butylamino)- 8-hydroxy-9H-purin-9-yl) methyl) aniline

Working concentration: 0.5 ng - 1 µg/ml (~1 nM - 10 µM)

Solubility: 828 g/mol

Formula: C41H65N9O7S

Molecular weight: 828 g/mol

Endotoxin level: <0.001 EU/µg

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Contents

  • 500 µg CL572

room temperature Products are shipped at room temperature.

store Stored at -20°C.

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Description

CL572 structure

CL572

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Details

HEK-Blue™ hTLR2 cells stimulation

HEK-Blue™ hTLR2 cells  which stably express an NF-κB-inducible SEAP reporter gene and human TLR2 were incubated in HEK-Blue™ Detection (a SEAP detection growth medium) and stimulated with increasing concentrations of the agonists indicated in the graph.
After 24h incubation, the levels of NF-κB-induced SEAP were determined by reading the OD at 655 nm.

HEK-Blue™ hTLR7 cells stimulation

HEK-Blue™ hTLR7 cells, which stably express an NF-κB-inducible SEAP reporter gene and human TLR7 were incubated in HEK-Blue™ Detection (a SEAP detection growth medium) and stimulated with increasing concentrations of the agonists indicated in the graph.
After 24h incubation, the levels of NF-κB-induced SEAP were determined by reading the OD at 655 nm.

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