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CL531

CL531 Unit size Cat. code Docs Qty Price
TLR2 & TLR7 Ligand
500 µg
tlrl-c531
+-
$142.00

CL531 (S-(2,3-bis(palmitoyloxy)-(2RS)propyl)-(R)-cysteinyl-(S)-seryl-(S)-lysyl-Ne- (4-((6-amino-2-(butylamino)-8-hydroxy-9H-purin-9-yl)methyl) benzylamido)(S)- lysyl-(S)-lysyl-(S)-lysine) is an 8-hydroxyadenine derivative conjugated to the lateral chain of the second lysine of Pam2CSK4.

CL531 is a very potent TLR2 agonist and a good TLR7 agonist. TLR2-mediated activation of NF-κB is achieved with concentrations as low as 5 pM (0.01 ng/ml).

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Specifications

Specificity: TLR2 and TLR7 agonist

Synonym: S-(2,3-bis(palmitoyloxy)- (2RS)propyl)-(R)- cysteinyl-(S)- seryl-(S)- lysyl-Ne- (4- ((6-amino-2-(butylamino)-8-hydroxy-9Hpurin- 9-yl) methyl) benzylamido) (S)-lysyl-(S)- lysyl-(S)-lysine

Working concentration: 5 pg - 10 µg/ml (~3pM - 10 µM)

Solubility: H2O (1 mg/ml)

Formula: C82H144N16O14S

Molecular weight: 1610 g/mol

Endotoxin level: <0.001 EU/mg

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Contents

  • 500 µg CL531
  • 1.5 ml sterile endotoxin-free water

room temperature Products are shipped at room temperature.

store Stored at -20°C.

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Description

CL531 chemical structure

CL531

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Details

HEK-Blue™ hTLR2 cells stimulation

HEK-Blue™ hTLR2 cells  which stably express an NF-κB-inducible SEAP reporter gene and human TLR2 were incubated in HEK-Blue™ Detection (a SEAP detection growth medium) and stimulated with increasing concentrations of the agonists indicated in the graph.
After 24h incubation, the levels of NF-κB-induced SEAP were determined by reading the OD at 655 nm.

HEK-Blue™ hTLR7 cells stimulation

HEK-Blue™ hTLR7 cells, which stably express an NF-κB-inducible SEAP reporter gene and human TLR7 were incubated in HEK-Blue™ Detection (a SEAP detection growth medium) and stimulated with increasing concentrations of the agonists indicated in the graph.
After 24h incubation, the levels of NF-κB-induced SEAP were determined by reading the OD at 655 nm.

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