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CL419

CL419 Unit size Cat. code Docs Qty Price
TLR2 ligand & nucleic acid carrier
500 µg
tlrl-c419
+-
$142.00

TLR2 Ligand & Nucleic Acid Carrier

CL419 (S-(2,3-bis(palmitoyloxy)-(2RS)propyl)-(R)-cysteinyl spermine) is a polyamine TLR2 agonist derived from Pam2CSK4 by replacement of Ser-(Lys)4 by a cationic sperminyl group.

CL419 forms positively charged liposomes which allows it to complex nucleic acids and transport them into the cytosol and the nucleus. CL419 / nucleic acid complexes are recognized by TLR2 and nucleic acid sensors leading to the significant activation of the NF-kB and IRF pathways.

In vivo, CL419 complexed with a plasmid DNA (pDNA) and injected intratumorally induces a modest reduction of the tumor growth.

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Specifications

Specificity: TLR2 and nucleic acid carrier

Synonym: S-(2,3-bis(palmitoyloxy)-(2RS)propyl)-(R)-cysteinyl spermine

Working concentration: 1 ng - 100 µg/ml (~1 nM - 100 µM)

Solubility: H2O (1 mg/ml)

Formula: C48H97N5O5S

Molecular weight: 856 g/mol

Endotoxin level: <0.001 EU/µg

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Contents

  • 500 µg CL419
  • 1.5 ml sterile endotoxin-free water

room temperature Products are shipped at room temperature.

store Stored at -20°C.

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Description

CL419 structure

CL419

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Details

HEK-Blue™ hTLR2 cells stimulation

HEK-Blue™ hTLR2 cells  which stably express an NF-κB-inducible SEAP reporter gene and human TLR2 were incubated in HEK-Blue™ Detection (a SEAP detection growth medium) and stimulated with increasing concentrations of the agonists indicated in the graph.
After 24h incubation, the levels of NF-κB-induced SEAP were determined by reading the OD at 655 nm.

RAW-Blue™ cells, which stably express an NF-kB-inducible SEAP reporter gene, were stimulated with 0.6 mg/ml of multi-PRR ligands

RAW-Blue™ cells, which stably express an NF-κB-inducible SEAP reporter gene, were stimulated with 0.6 µg/ml of InvivoGen’s multi-PRR ligands complexed with 0.1 µg/ml HSV-60 (synthetic dsDNA).
After 24h incubation, the levels of NF-κB-induced SEAP were determined using QUANTI-Blue™, a SEAP detection reagent.

Stimulation of RAW-Lucia cells

RAW-Lucia cells, which stably express an IRF-inducible Lucia, a secreted luciferase reporter gene, were stimulated with 6 µg/ml of InvivoGen’s multi-PRR ligands complexed with 1 µg/ml HSV-60 (synthetic dsDNA). After 24h incubation, the levels of IRF-induced Lucia luciferase were determined using QUANTI-Luc™, a Lucia luciferase detection reagent.

 Antitumor effect of CL419, CL347 & CL553

(A) Tumor growth after treatment with CL419, CL347 or CL401complexed with plasmid DNA (pDNA). Seven days after C57BL/6 mice were inoculated subcutaneously with 5.10e5 B16-F1 cells, pDNA/CL419, pDNA/CL347 or pDNA/CL553 complexes were injected intratumorally at a 10:40 (w:w) ratio (10 µg:40 µg/mouse/100 µl) on days 7 and 16. A fourth group received intratumoral injections of the vehicle.

(B) Survival curves for untreated as well as pDNA/CL419-, pDNA/CL347- or pDNA/CL553-treated mice.
Each group contained 8 mice. Black arrows represent the days of injection. Tumor growth was monitored and measured with calipers after day 5 of grafting tumor cells into mice and then every 2 days thereafter. Tumor volume in mm3 was determined according to the formula V = W2 x L/2, where L = length (mm) and W = width (mm).

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