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CL401

CL401 Unit size Cat. code Docs Qty Price
TLR2 & TLR7 Ligand
500 µg
tlrl-c401
+-
$142.00

TLR2 & TLR7 Ligand

CL401 (S-(2,3-bis(palmitoyloxy)-(2RS)propyl)-(R)-cysteinyl 4-((6-amino-2(butyl amino)-8-hydroxy-9H-purin-9-yl)methyl) aniline) is a small lipophilic molecule comprising an 8-hydroxyadenine compound conjugated with a Pam2C group.

This bipartite structure confers to CL401 the ability to efficiently stimulate both TLR7 and TLR2, respectively. IIntratumoral injection of CL401 leads to a significant antitumor activity (see Details).

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Specifications

Specificity: TLR2 and TLR7 agonist

Synonym: S-(2,3-bis(palmitoyloxy)-(2RS)propyl)-(R)-cysteinyl 4- ((6-amino-2(butyl amino)- 8-hydroxy-9H- purin-9-yl) methyl) aniline

Working concentration: 1 ng- 10 µg/ml (~1nM - 10 µM)

Solubility: DMSO (20 mg/ml)

Endotoxin level:

Formula: C54H92N8O6S

Molecular weight: 981g/mol

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Contents

  • 500 µg CL401
  • 2 ml sterile endotoxin-free water

room temperature Products are shipped at room temperature

store Stored at -20°C.

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Description

CL401 chemical structure

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Details

HEK-Blue™ hTLR2 cells stimulation

HEK-Blue™ hTLR2 cells  which stably express an NF-κB-inducible SEAP reporter gene and human TLR2 were incubated in HEK-Blue™ Detection (a SEAP detection growth medium) and stimulated with increasing concentrations of the agonists indicated in the graph.
After 24h incubation, the levels of NF-κB-induced SEAP were determined by reading the OD at 655 nm.

HEK-Blue™ hTLR7 cells stimulation

HEK-Blue™ hTLR7 cells, which stably express an NF-κB-inducible SEAP reporter gene and human TLR7 were incubated in HEK-Blue™ Detection (a SEAP detection growth medium) and stimulated with increasing concentrations of the agonists indicated in the graph.
After 24h incubation, the levels of NF-κB-induced SEAP were determined by reading the OD at 655 nm.

RAW-Blue™ cells, which stably express an NF-kB-inducible SEAP reporter gene, were stimulated with 0.6 mg/ml of multi-PRR ligands

RAW-Blue™ cells, which stably express an NF-κB-inducible SEAP reporter gene, were stimulated with 0.6 µg/ml of InvivoGen’s multi-PRR ligands complexed with 0.1 µg/ml HSV-60 (synthetic dsDNA).
After 24h incubation, the levels of NF-κB-induced SEAP were determined using QUANTI-Blue™, a SEAP detection reagent.

Antitumor effect of CL401 & CL413

(A) Tumor growth after CL413 or CL401 treatment. C57BL/6 mice were inoculated subcutaneously with 5x10e5 B16-F1 mouse melanoma cells. After seven days, CL413, CL401 or vehicle were injected intratumorally (50 µg/mouse/50 µl) on days 5, 15 and 20. (B) Survival curves for untreated as well as CL413- or CL401-treated mice.

Each group contained 8 mice. Black arrows represent the days of injection. Tumor growth was monitored and measured with calipers after day 5 of grafting tumor cells into mice and then every 2 days thereafter. Tumor volume in mm3 was determined according to the formula V = W2 x L/2, where L = length (mm) and W = width (mm).

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