c-di-UMP

STING agonist negative control

Cyclic di-uridine monophosphate (c-di-UMP) is a synthetic cyclic dinucleotide containing a pyrimidine base.

Unlike the purine-containing cyclic dinucleotides (c-di-AMP, c-di-GMP and c-di-IMP), c-di-UMP is not predicted to bind strongly to STING [1]. The smaller size of the pyrimidine base is expected to prevent the formation of stacking interactions that are crtitical for proper binding to STING.

Indeed, STING-expressing cells, such as THP1-Blue™ ISG cells, do not respond to c-di-UMP, even at high concentrations.

Reference:

1. Yin Q. et al., 2012. Cyclic di-GMP sensing via the innate immune signaling protein STING. Mol Cell. 46(6):735-45.

Specifications

Description: STING agonist negative control

Synonym: Cyclic di-uridine monophosphate, c-di-UMP sodium salt

CAS number: 73120-97-5

Formula: C₁₈H₂₀N₄O₁₆P₂ .2Na

Molecular weight: 656.3

Purity: ≥ 95% by LC/MS

Solubility: 50 mg/ml in water

Quality control:

• The inability of c-di-UMP to induce the STING/TBK1/IRF3 pathway has been confirmed in THP1-Blue™ ISG cells.
• The absence of endotoxins and other bacterial contaminants has been confirmed using HEK-Blue™ TLR4 and HEK-Blue™ TLR2 cells.

Contents

• 1 mg lyophilized c-di-UMP
• 1.5 ml endotoxin-free water

Product is shipped at room temperature

Stored at -20°C.

Product is sterile filtered prior to lyophilization.