c-di-IMP

STING ligand - Cyclic [I(3’,5’)pI(3’,5’)p]

Cyclic di-inosine monophosphate (c-di-IMP) is a synthetic analog of the bacterial second messengers c-di-AMP and c-di-GMP.
Although chemically different, c-di-IMP exhibits similar conformational features and biological properties to c-di-GMP. In vitro studies have revealed that c-di-IMP displays comparable capacities to c-di-GMP in promoting the activation and maturation of antigen presenting cells [1]. Furthermore, c-di-IMP has been shown to possess strong adjuvant properties when co-administered with an antigen by the mucosal route [1].

The immunostimulatory activity of c-di-IMP depends on the signaling molecule STING [2]. Cells with reduced STING expression respond weakly to c-di-IMP in contrast to cells expressing high levels of STING.

References:

1. Libanova R. et al., 2010.The member of the cyclic di-nucleotide family bis-(3', 5')-cyclic dimeric inosine monophosphate exer ts potent activity as mucosal adjuvant. Vaccine. 28(10):2249-58.
2. Burdette DL. et al., 2011. STING is a direct innate immune sensor of cyclic di-GMP. Nature. 478(7370):515-8.

Specifications

Description: STING Agonist

Synonym: Cyclic di-inosine monophosphate, c-di-IMP sodium salt

CAS number: 79940-41-3

Formula: C₂₀H₂₀N₈O₁₄P₂ .2Na

Molecular weight: 704.35

Purity: ≥ 95% by LC/MS

Solubility: 50 mg/ml in water

Quality control:

• The biological activity is assessed by measuring induction of the interferon pathway in THP1-Blue™ ISG cells.
• The absence of endotoxins and other bacterial contaminants has been confirmed using HEK-Blue™ TLR4 and HEK-Blue™ TLR2 cells.

Contents

• 1 mg lyophilized c-di-IMP
• 1.5 ml endotoxin-free water

Product is shipped at room temperature.

Product should be stored at -20 °C.

Product is sterile filtered prior to lyophilization.