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c-di-GMP Control

c-di-GMP Control Unit size Cat. code Docs Qty Price
STING agonist negative control
1 mg
tlrl-napgpg
+-
$115.00

STING agonist negative control

c-di-GMP Control, also known as 5'-pGpG, is a linear dinucleotide analog that can be obtained after hydrolysis of cyclic diguanylate monophosphate (c-di-GMP) by phosphodiesterases [1]. c-di-GMP is a cyclic dinucleotide (CDN) produced in bacteria in which it functions as a second messenger. c-di-GMP is involved in complex biological processes, such as biofilm formation, virulence and photosynthesis.

CDNs including c-di-GMP bind the cytosolic DNA sensor STING (stimulator of interferon genes) and induce the production of type I interferons (IFNs). Due to its linear conformation, 5'-pGpG is intended to serve as a negative control for c-di-GMP in type I IFN induction assays. The importance of dinucleotide conformation has been well established in bacteria, where RNA regulatory riboswitches are able to discriminate between biologically active CDNs and their corresponding linear dinucleotides [2-4].

 

1. Römling U. et al., 2013. Cyclic di-GMP: the First 25 Years of a Universal Bacterial Second Messenger. Microbiol Mol Biol Rev. 77(1): 1–52.
2. Ren A. et al., 2015. Structural Basis for Molecular Discrimination by a 3',3'-cGAMP Sensing Riboswitch. Cell Rep. 11(1):1-12.
3. Gao J. et al., 2015. Identification and characterization of phosphodiesterases that specifically degrade 3'3'-cyclic GMP-AMP. Cell Res. 25(5):539-50.
4. Witte CE. et al., 2013. Cyclic di-AMP is critical for Listeria monocytogenes growth, cell wall homeostasis, and establishment of infection. MBio. 4(3):e00282-13.

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Specifications

Description: c-di-GMP negative control

Synonym: 5'-pGpG sodium salt

CAS number: 33008-99-0

Formula: C20H23N10O15P2 .3Na

Molecular weight: 774.38

Purity: ≥ 95% by LC/MS

Solubility: 50 mg/ml in water

Quality control:

  • The inability of c-di-GMP Control to induce type I interferon (IFN) has been confirmed in THP1-Blue™ ISG cells.
  • The absence of endotoxins and other bacterial contaminants has been confirmed using HEK-Blue™ TLR4 and HEK-Blue™ TLR2 cells.
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Contents

  • 1 mg lyophilized c-di-GMP Control
  • 1.5 ml endotoxin-free water

room temperature Product is shipped at room temperature

store Stored at -20 °C.

Product is sterile filtered prior to lyophilization.

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