c-di-AMP Control

STING agonist negative control

c-di-AMP Control, also known as 5'-pApA, is a linear dinucleotide analog that can be obtained after hydrolysis of cyclic diadenylate monophosphate (c-di-AMP) by phosphodiesterases [1]. c-di-AMP is a cyclic dinucleotide (CDN) produced in bacteria in which it functions as a second messenger. CDNs including c-di-AMP bind the cytosolic DNA sensor STING (stimulator of interferon genes) and induce the production of type I interferons (IFNs). Due to its linear conformation, 5'-pApA is intended to serve as a negative control for c-di-AMP in type I IFN induction assays.

The importance of dinucleotide conformation has been well established in bacteria, where RNA regulatory riboswitches are able to discriminate between biologically active CDNs and their corresponding linear dinucleotides [2-4].
 

References:

1. Huynh TN. & Woodward JJ, 2016. Too much of a good thing: regulated depletion of c-di-AMP in the bacterial cytoplasm. Curr Opin Microbiol. 30:22-29.
2. Ren A. et al., 2015. Structural Basis for Molecular Discrimination by a 3',3'-cGAMP Sensing Riboswitch. Cell Rep. 11(1):1-12.
3. Gao J. et al., 2015. Identification and characterization of phosphodiesterases that specifically degrade 3'3'-cyclic GMP-AMP. Cell Res. 25(5):539-50.
4. Witte CE. et al., 2013. Cyclic di-AMP is critical for Listeria monocytogenes growth, cell wall homeostasis, and establishment of infection. MBio. 4(3):e00282-13.

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Specifications

Description: c-di-AMP negative control

Synonym: 5'-pApA sodium salt

CAS number: 3593-47-3

Formula: C₂₀H₂₃N₁₀O₁₂P₂ .2Na

Molecular weight: 742.38

Purity: ≥ 95% by LC/MS

Solubility: 50 mg/ml in water

Quality control:

• The inability of c-di-AMP Control to induce type I interferon (IFN) has been confirmed in THP1-Blue™ ISG cells.
• The absence of endotoxins and other bacterial contaminants has been confirmed using HEK-Blue™ TLR4 and HEK-Blue™ TLR2 cells.

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Contents

• 1 mg lyophilized c-di-AMP Control
• 1.5 ml endotoxin-free water

Product is shipped at room temperature .

Stored at -20 °C.

Product is sterile filtered prior to lyophilization.

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