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B-Class TLR9 Agonist Kit

B-Class TLR9 Agonist Kit Unit size Cat. code Docs Qty Price
Set of known multispecies TLR9 agonists (B-Class CpG ODNs)
1 kit
tlrl-kit9b
+-
$405.00

Set of known multispecies TLR9 agonists (B-Class CpG ODNs)

CpG ODNs of this kit belong exclusively to the B class and are active in human and/or mouse and other species.

The kit contains prototype CpG ODNs as well as less popular but worth testing CpG ODNs.

- ODN 1668 and ODN 1826 - B-class, mouse preferred
- ODN 2006 - B class, human preferred
- ODN BW006 - B class, human/mouse
- ODN 2007 - B-class, bovine/porcine
- ODN D-SL01 - B-class, multispecies

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Specifications

Specificity: TLR9 agonists

Quality control
- TLR9 activity has been tested using HEK-Blue™ TLR9 cells.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.

Sequences
ODN 1668 (mouse preferred): 5’-tccatgacgttcctgatgct-3’ (20 mer)
ODN 1826 (mouse preferred): 5’-tccatgacgttcctgacgtt-3’ (20 mer)
ODN 2006 (human preferred): 5’-tcgtcgttttgtcgttttgtcgtt-3’ (24 mer)
ODN BW006 (human/mouse): 5’-tcgacgttcgtcgttcgtcgttc-3’ (23 mer)
ODN 2007 (bovine/porcine): 5’-tcgtcgttgtcgttttgtcgtt-3’ (22 mer)
ODN D-SL01 (multispecies): 5’-tcgcgacgttcgcccgacgttcggta-3’ (26 mer)
Note: Bases shown in capital letters are phosphodiester and those in lower case are phosphorothioate (nuclease resistant).

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Contents

ODNs are provided lyophilized:
• 100 μg (15.71 nmol) ODN 1668
• 100 μg (15.71 nmol) ODN 1826
• 100 μg (12.98 nmol) ODN 2006 (ODN 7909 or PF-3512676)
• 100 μg (14.16 nmol) ODN 2007
• 100 μg (13.62 nmol) ODN BW006 (ODN 684)
• 100 μg (11.99 nmol) ODN D-SL01
• 1.5 ml endotoxin-free water

Products are shipped at room temperature and should be stored at -20°C.

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Description

CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs). These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA [1, 2].

CpG ODNs are recognized by Toll-like receptor 9 (TLR9) leading to strong immunostimulatory effects.
Three classes of stimulatory CpG ODNs have been identified, classes A, B and C, which differ in their immune-stimulatory activities [3-4]. Class B CpG ODNs contain a full phosphorothioate backbone with one or more CpG dinucleotides. They strongly activate B cells but weakly stimulate IFN-α secretion.

CpG ODNs of this kit belong exclusively to the B class and are active in human and/or mouse and other species.
The kit contains prototype CpG ODNs as well as less popular CpG ODNs.

• ODN 1668 has a preference towards mouse TLR9.

• ODN 1826 is a class B prototype ODN with a preference towards mouse TLR9.

• ODN 2006 (also known as ODN 7909 or PF-3512676) is a class B prototype ODN with a preference towards human TLR9.

• ODN BW006 (also known as ODN 684) is capable of activating human and mouse TLR9 as vigorously as ODN 2006.

• ODN 2007 has a preference towards bovine and porcine TLR9.

• ODN D-SL01 (double-stem loop ODN) is a TLR9 agonist in a diverse vertebrate species (humans, mice, rats, rabbits, pigs, swine and dogs).

1. Krieg am. et al., 1995. CpG motifs in bacterial DNA trigger direct B-cell activation. Nature, 374(6522):546-9.
2. Bauer, S. et al., 2001. Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. PNAS 98(16):9237-42.
3. Krug a. et al., 2001. Identification of CpG oligonucleotide sequences with high induction of IFNalpha/beta in plasmacytoid dendritic cells. Eur J Immunol, 31(7): 2154-63.
4. marshall JD. et al., 2005. Superior activity of the type C class of ISS in vitro and in vivo across multiple species. DNA Cell Biol. 24(2):63-72.

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