Anti-hCD14-IgA [ Discontinued ]
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Human IgA2 against human CD14
October, 18th 2017
The IgA version of this anti-hCD14 antibody has been replaced by an IgG isotype (Anti-hCD14-IgG).
Anti-hCD14-IgA is a chimeric monoclonal antibody specific for human CD14.
It was generated by combining the constant domains of the human IgA molecule with murine variable regions.
This antibody has been selected for its ability to efficiently neutralize the biological activity of TLR2 or TLR4.
Clonality: Monoclonal antibody.
Applications: Neutralizing TLR2 or TLR4-induced cellular activation. It can also be used for flow cytometry.
Specificity: Human CD14.
Isotype: Human IgA2.
Formulation: 0.2 μm filtered solution in Tris HCl with glycine, saccharose and stabilizing agents.Back to the top
- 100 μg purified anti-hCD14-IgA antibody, provided azide-free and lyophilized.
Product is shipped at room temperature.
Store lyophilized antibody at -20 °C.
Lyophilized product is stable for at least 1 year.Back to the top
CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein which acts as a bacterial pattern recognition receptor .
CD14 is found on cells derived from the monocyte/macrophage lineage, as well as neutrophils and B lymphocytes.
CD14 serves as a member of the heteromeric lipopolysaccharide (LPS) receptor complex that also contains TLR4 and MD2 .
CD14 binds LPS but is not capable of initiating a transmembrane activation signal since it does not contain a cytoplasmic domain.
Upon LPS binding, CD14 physically associates with TLR4 which in turn transduces the signal. CD14 was also shown to interact with TLR2 in response to various microbial infections .
1. Pugin J. et al., 1994. CD14 is a pattern recognition receptor. Immunity. 1(6):509-16.
2. Da Silva Correia J. et al., 2001. Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2. J Biol Chem. 276(24): 21129- 35.
3. Aderem A. & Ulevitch R.J. , 2000. Toll-like receptors in the induction of the innate immune response. Nature. 406(6797):782-7.