Anti-FLA-ST Unit size Cat. code Docs Price
Detection and Neutralizing antibody to flagellin from S. typhimurium
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January 20th, 2020.
This antibody has been removed from our catalog. However, you may contact us if you wish to order minimal quantities.

Detection and neutralization antibody to flagellin from S. typhimurium

Anti-FLA-ST is a mouse monoclonal antibody specific against flagellin from Salmonella typhimurium.

The antibody has been screened for the ability to neutralize FLA-ST-induced TLR5 activity. Anti-FLA-ST can be used for detection by ELISA and neutralization of purified or recombinant S. typhimurium flagellin.

The utility of this antibody for other applications has not been determined.

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Clonality: Monoclonal antibody

Clone: 18E11

Isotype: Mouse IgG1, kappa

Formulation: 0.2 µm filtered solution in 68 mM phosphate buffer with 91 mM glycine, 5% w/v saccharose and stabilizing agents

Application: Neutralization of FLA-ST-induced TLR5 activity, detection by ELISA

Specificity: flagellin from Salmonella typhimurium

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  • 100 µg purified anti-FLA-ST provided azide-free and lyophilized

Room temperature Product is shipped at room temperature

Store Store lyophilized antibody at -20°C.

Stability Product is stable for 6 months.

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Neutralization activity of hTLR5-Fc

Neutralization activity of hTLR5-Fc:
Increasing concentrations of hTLR5-Fc, anti-FLA-ST (antibody against S. typhimurium flagellin) or anti-hTLR5 (antibody against human TLR5) were pre-incubated with 5 µg FLA-ST (S. typhimurium flagellin) prior to the addition of HEK-Blue™ hTLR5 cells, which express the human TLR5 gene and an NF-kB-inducible SEAP gene.
After 24h incubation, TLR5-induced NF-kB activation was assessed by measuring the levels of SEAP using the QUANTI-Blue™ assay.

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