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Detection and Neutralizing antibody to flagellin from S. typhimurium
Detection and neutralization antibody to flagellin from S. typhimurium
Anti-FLA-ST is a mouse monoclonal antibody specific against flagellin from Salmonella typhimurium.
The antibody has been screened for the ability to neutralize FLA-ST-induced TLR5 activity. Anti-FLA-ST can be used for detection by ELISA and neutralization of purified or recombinant S. typhimurium flagellin.
The utility of this antibody for other applications has not been determined.Back to the top
Clonality: Monoclonal antibody
Isotype: Mouse IgG1
Formulation: 0.2 µm filtered solution in 68 mM phosphate buffer with 91 mM glycine, 5% w/v saccharose and stabilizing agents
Application: Neutralization of FLA-ST-induced TLR5 activity, detection by ELISA
Specificity: flagellin from Salmonella typhimuriumBack to the top
- 100 µg purified anti-FLA-ST provided azide-free and lyophilized
Product is shipped at room temperature
Store lyophilized antibody at -20°C.
Product is stable for 6 months.Back to the top
Neutralization activity of hTLR5-Fc:
Increasing concentrations of hTLR5-Fc, anti-FLA-ST (antibody against S. typhimurium flagellin) or anti-hTLR5 (antibody against human TLR5) were pre-incubated with 5 µg FLA-ST (S. typhimurium flagellin) prior to the addition of HEK-Blue™ hTLR5 cells, which express the human TLR5 gene and an NF-kB-inducible SEAP gene.
After 24h incubation, TLR5-induced NF-kB activation was assessed by measuring the levels of SEAP using the QUANTI-Blue™ assay.