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Anti-FLA-ST

Anti-FLA-ST Unit size Cat. code Docs Qty Price
Detection and Neutralizing antibody to flagellin from S. typhimurium
100 µg
mabg-flast
+-
$325.00

Detection and neutralization antibody to flagellin from S. typhimurium

Anti-FLA-ST is a mouse monoclonal antibody specific against flagellin from Salmonella typhimurium.

The antibody has been screened for the ability to neutralize FLA-ST-induced TLR5 activity. Anti-FLA-ST can be used for detection by ELISA and neutralization of purified or recombinant S. typhimurium flagellin. The utility of this antibody for other applications has not been determined.

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Specifications

Clonality: Monoclonal antibody
Clone:
18E11
Isotype:
Mouse IgG1
Formulation:
0.2 µm filtered solution in 68 mM phosphate buffer with 91 mM glycine, 5% w/v saccharose and stabilizing agents
Application: Neutralization of FLA-ST-induced TLR5 activity, detection by ELISA
Specificity: flagellin from Salmonella typhimurium

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Contents

- 100 µg purified anti-FLA-ST provided azide-free and lyophilized

Product is shipped at room temperature.
Store lyophilized antibody at -20°C. Product is stable for 6 months.

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Details

Neutralization activity of hTLR5-Fc

Neutralization activity of hTLR5-Fc:
Increasing concentrations of hTLR5-Fc, anti-FLA-ST (antibody against S. typhimurium flagellin) or anti-hTLR5 (antibody against human TLR5) were pre-incubated with 5 µg FLA-ST (S. typhimurium flagellin) prior to the addition of HEK-Blue™ hTLR5 cells, which express the human TLR5 gene and an NF-kB-inducible SEAP gene.
After 24h incubation, TLR5-induced NF-kB activation was assessed by measuring the levels of SEAP using the QUANTI-Blue™ assay.

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