AG490

JAK2 Inhibitor

AG490 is a specific and potent inhibitor of the Janus kinase 2 protein (JAK2) [1]. JAK2 regulates the phosphorylation of JNK, primarily through PI3K. It has been established that JAK2 plays an important role in TLR-mediated biological responses, blocking TLR4-mediated responses to LPS [2,3] and TLR5-mediated responses to flagellin [4]. JAK2 is also involved in cytokine signaling, such as IL-2, IL-6 and IL-12.

References:

1. Levitzki A. 1990. Tyrphostins- potential antiprolierative agents and novel molecular tools. Biochem. Pharmacol. 40:913-918.
2. Okugawa S. et al., 2003. Janus kinase 2 is involved in lipopolysaccaride-induced activation of macrophages. Am. J. Physiol. Cell Physiol. 285: C399-C408.
3. Kimura A et al. 2005. Suppressor of cytokine signaling-1 selectively inhibits LPSinduced IL-6 production by regulating JAK–STAT. PNAS 102:17089-17094
4. Ha H et al. 2008. Stimulation by TLR5 Modulates Osteoclast Differentiation through STAT1/IFN-β1. J. Immuno.180:1382-1389.

Specifications

Working concentration: 20-50 µM

Purity: ≥99% (UHPLC)

CAS number: 133550-30-8

Synonym: Tyrphostin B42

Formula: C₁₇H₁₄N₂O₃

Molecular weight: 294.3

Solubility: 50 mg/ml in DMSO and 5 mg/ml in ethanol

Quality control: The absence of bacterial contamination (e.g. lipoproteins and endotoxins) is confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.

Contents

AG490 is provided lyophilized.

• 10 mg AG490

AG490 is shipped at room temperature.

Store at -20 °C.