A & C-Classes TLR9 Agonist Kit

A & C-Classes TLR9 Agonist Kit Unit size Cat. code Docs Qty Price
Set of known multispecies TLR9 agonists (A & C-Classes CpG ODNs)
1 kit

Set of known multispecies TLR9 agonists (A & C-Classes CpG ODNs)

This kit contains CpG ODNs that belong to the A or C class. They are active in several species.

- ODN 1585 - A-class, mouse preferred
- ODN 2216 and ODN 2336 - A class, human preferred
- ODN 2395 and ODN M362 - C-class, human/mouse
- ODN D-SL03 - C-class multispecies

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Specificity: TLR9 agonists

Quality control
- TLR9 activity has been tested using HEK-Blue™ TLR9 cells.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.

ODN 1585 (mouse preferred): 5’-ggGGTCAACGTTGAgggggg-3’ (20 mer)
ODN 2216 (human/mouse): 5’-ggGGGACGA:TCGTCgggggg-3’ (20 mer)
ODN 2336 (human/mouse): 5’-gggGACGAC:GTCGTGgggggg-3’ (21 mer)
ODN 2395 (human preferred): 5’-tcgtcgttttcggcgc:gcgccg-3’ (22 mer)
ODN M362 (human preferred): 5’-tcgtcgtcgttc:gaacgacgttgat-3’ (25 mer)
ODN D-SL03 (multispecies): 5’-tcgcgaacgttcgccgcgttcgaacgcgg-3’ (29 mer)
Note: Bases shown in capital letters are phosphodiester and those in lower case are phosphorothioate (nuclease resistant). Palindrome is underlined.

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ODNs are provided lyophilized:
• 100 μg (15.51 nmol) ODN 1585
• 100 μg (15.54 nmol) ODN 2216
• 100 μg (14.75 nmol) ODN 2336
• 100 μg (14.18 nmol) ODN 2395
• 100 μg (12.40 nmol) ODN M362
• 100 μg (10.7 nmol) ODN D-SL03
• 1.5 ml endotoxin-free water

Products are shipped at room temperature and should be stored at -20°C.

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CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs) that are recognized by Toll-like receptor 9 (TLR9) leading to strong immunostimulatory effects.

Three classes of stimulatory CpG ODNs have been identified, classes A, B and C, which differ in their immunostimulatory activities [1, 2].
Class A CpG ODNs are characterized by a phosphodiester central CpG-containing palindromic motif and a phosphorothioate 3’ poly-G string. They induce high IFN-α production from plasmacytoid dendritic cells (pDC) but are weak stimulators of TLR9-dependent NF-kB signaling.
Class C CpG ODNs contain a complete phosphorothioate backbone and a CpG-containing palindromic motif. Class C CpG ODNs are potent inducers of IFN-α from pDC and strong B cell activators [3, 4].

This kit contains CpG ODNs that belong to the A or C class. They are active in several species.

• ODN 1585 is a A-class CpG ODN with a preference towards mouse TLR9.

• ODN 2216 & ODN 2336 are A-class CpG ODN with a preference towards human and mouse TLR9.

• ODN 2395 & ODN M362 are C-class CpG ODN with a preference towards human TLR9.

• ODN D-SL03 (double-stem loop ODN) is a C-class CpG ODN and a TLR9 agonist in a diverse vertebrate species (humans, mice, rats, rabbits, pigs, swine and dogs).

1. Krug a. et al., 2001. Identification of CpG oligonucleotide sequences with high induction of IFNalpha/beta in plasmacytoid dendritic cells. Eur J Immunol, 31:2154-63.
2. Marshall J. et al., 2005. Superior activity of the type C class of ISS in vitro and in vivo across multiple species. DNA Cell Biol. 24:63-72.
3. Jurk M. et al., 2004. C-Class CpG ODN: sequence requirements and characterization of immunostimulatory activities on mRNA level. Immunobiology. 209:141-54.
4. Abel K. et al., 2005. Deoxycytidyl-deoxyguanosine oligonucleotide classes A, B, and C induce distinct cytokine gene expression patterns in rhesus monkey peripheral blood mononuclear cells and distinct alpha interferon responses in TLR9-expressing rhesus monkey plasmacytoid dendritic cells. Clin Diagn Lab Immunol. 12:606-21.

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