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3'3'-cGAMP VacciGrade™

3’3’-cGAMP VacciGrade™ Unit size Cat. code Docs Qty Price
STING ligand - Cyclic [G(3’,5’)pA(3’,5’)p]
1 mg
vac-nacga
+-
$486.00

Cyclic [G(3’,5’)pA(3’,5’)p] - STING ligand

3’3’-cGAMP (cyclic [G(3’,5’)pA(3’,5’)p]) is a cyclic dinucleotide (CDN) produced by bacteria in which it serves as a second messenger and plays a role in chemotaxis and colonization [1]. 3’3’-cGAMP is also referred to as “canonical” cGAMP due the presence of the classical 3’-5’ phosphodiester linkages between the guanosine and the adenosine.

CDNs are a relatively new class of adjuvants that have been shown to increase vaccine potency [2]. CDNs activate innate immunity by directly binding to the endoplasmic reticulum-resident receptor STING (stimulator of interferon genes), activating a signaling pathway that induces the expression of interferon-β (IFN-β) and also nuclear factor-κB (NF-κB) dependent inflammatory cytokines [3]

3’3’-cGAMP VacciGrade™ is a preclinical grade preparation of the cyclic dinucleotide 3’3’-cGAMP.

 

3’3’-cGAMP is VacciGrade™ (preclinical grade) for research use only, not for use in humans.

 

1. Davies B. et al., 2012. Coordinated regulation of accessory genetic elements produces cyclic di-nucleotides for V. cholerae virulence. Cell. 149(2):358-70.
2. Dubensky TW. et al., 2013. Rationale, progress and development of vaccines utilizing STING-activating cyclic dinucleotide adjuvants. Therapeutic Advances in Vaccines 1(4): 131-143.
3. Zhang X. et al., 2013. Cyclic GMP-AMP containing mixed phosphdiester linkages is an endogenous high-affinity ligand for STING. Mol Cell.51(2):226-35.

IRF INDUCTION (Lucia luciferase reporter)
IRF INDUCTION (Lucia luciferase reporter)

THP1-Dual™ cells were stimulated for 24 hours with the STING ligands as shown (all at 10 μg/ml). IRF induction was determined by measuring the relative light units (RLUs) in a luminometer using QUANTI‑Luc™, a Lucia luciferase detection reagent.


THP1-Dual™ cells were stimulated for 24 hours with the STING ligands as shown (all at 10 μg/ml). NF‑kB induction was determined using QUANTI‑Blue™, a SEAP detection reagent, and by reading the optical density (OD) at 655 nm. Non-induced cells (NI) have been included as a negative control.

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Specifications

Description: STING agonist VacciGrade™

Synonym: Cyclic [G(3’,5’)pA(3’,5’)p], cyclic GMP-AMP; c-GpAp sodium salt

CAS number: 849214-04-6

Formula: C20H22N10O13P2 .2Na

Molecular weight: 718.38

Purity: ≥ 95% by LC/MS

Solubility: 50 mg/ml in physiological water

Quality: Sterile

Endotoxin Level: Lower than 0.005 EU/μg

Biological activity: Assessed by measuring induction of the interferon pathway in THP1-Blue™ ISG cells

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Contents

  • 1 mg (2 x 500 µg) lyophilized 3’3’-cGAMP VacciGrade™
  • 10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

3'3'-cGAMP VacciGrade™ is shipped at room temperature.

3'3'-cGAMP VacciGrade™ should be stored at -20°C.

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Description

3’3’-cGAMP VacciGrade™ is a preclinical grade preparation of the cyclic dinucleotide 3’3’-cGAMP.

It is prepared under strict aseptic conditions and is tested for the presence of endotoxins.

3’3’-cGAMP VacciGrade™ is guaranteed sterile.

Its endotoxin level is lower than 0.005 EU/μg.

 

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Citations

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