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293XL cell line expressing the HA-tagged human TLR9 gene
3-7 x 10e6 cells
293XL/hTLR9-HA cells were obtained by stable transfection of HEK293XL cells with the pUNO-hTLR9-HA plasmid which expresses the human TLR9 gene fused at the 3'end to the influenza hemaglutinine (HA).
The control cell line for 293XL/hTLR9-HA cells is 293XL/null cells.
Antibiotic resistance: blasticidin
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-freeBack to the top
Shipped on dry iceBack to the top
TLR9 is expressed in the endoplasmic reticulum of resting cells in contrast to most TLRs that are located on the plasma membrane . TLR9 recognizes specific unmethylated CpG-ODN sequences, that distinguish bacterial DNA from mammalian DNA. TLR9 engages an intracellular signaling pathway that involves MyD88 leading to NF-κB translocation . TLR9 recognizes different CpG motifs; the optimal sequences being GTCGTT and GACGTT for human TLR9 and murine TLR9 respectively . TLR9 binds directly to CpG DNA and recognizes specifically CpG DNA that is unmethylated and single stranded .
1. Latz E. et al., 2004. TLR9 signals after translocating from the ER to CpG DNA in the lysosome. Nat Immunol. 5(2):190-8.
2. Chuang TH. et al., 2002. Toll-like receptor 9 mediates CpG-DNA signaling. J Leukoc Biol, 71(3):538-44.
3. Bauer S. et al., 2001. Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. Proc Natl Acad Sci USA, 98(16):9237-42.
4. Rutz M. et al., 2004. Toll-like receptor 9 binds single-stranded CpGDNA in a sequence- and pH-dependent manner. Eur J Immunol. 34(9):2541-50.