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293XL cell line expressing the HA-tagged human TLR9 gene
3-7 x 10e6 cells
293XL/hTLR9-HA cells were obtained by stable transfection of HEK293XL cells with the pUNO-hTLR9-HA plasmid which expresses the human TLR9 gene fused at the 3'end to the influenza hemaglutinine (HA).
The control cell line for 293XL/hTLR9-HA cells is 293XL/null cells.Back to the top
Antibiotic resistance: blasticidin
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-freeBack to the top
Shipped on dry ice (Europe, USA & Canada)Back to the top
TLR9 is expressed in the endoplasmic reticulum of resting cells in contrast to most TLRs that are located on the plasma membrane . TLR9 recognizes specific unmethylated CpG-ODN sequences, that distinguish bacterial DNA from mammalian DNA. TLR9 engages an intracellular signaling pathway that involves MyD88 leading to NF-κB translocation . TLR9 recognizes different CpG motifs; the optimal sequences being GTCGTT and GACGTT for human TLR9 and murine TLR9 respectively . TLR9 binds directly to CpG DNA and recognizes specifically CpG DNA that is unmethylated and single stranded .
1. Latz E. et al., 2004. TLR9 signals after translocating from the ER to CpG DNA in the lysosome. Nat Immunol. 5(2):190-8.
2. Chuang TH. et al., 2002. Toll-like receptor 9 mediates CpG-DNA signaling. J Leukoc Biol, 71(3):538-44.
3. Bauer S. et al., 2001. Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. Proc Natl Acad Sci USA, 98(16):9237-42.
4. Rutz M. et al., 2004. Toll-like receptor 9 binds single-stranded CpGDNA in a sequence- and pH-dependent manner. Eur J Immunol. 34(9):2541-50.