|293/hTLR6-HA||Unit size||Cat. code||Docs||Qty||Price|
HEK 293 cells stably transfected with the HA-tagged human TLR6 gene
3-7 x 10e6 cells
293/hTLR6-HA cells were obtained by stable transfection of HEK293 cells with the pUNO-hTLR6-HA plasmid which expresses the human TLR6 gene fused at the 3'end to the influenza hemaglutinine (HA).
The control cell line for 293/hTLR6-HA cells is 293/null.
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Antibiotic resistance: blasticidin
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-freeBack to the top
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TLR6, which is closely related to TLR1, is expressed predominantly in spleen, thymus, ovary and lung . TLR6 cooperates with TLR2 to recognize diacylated lipoproteins (LPs) found in mycoplasma but is not required in the recognition of triacylated LPs found in bacteria . This suggests that TLR6 is able to discriminate between the N-terminal lipoylated structures of LPs derived from mycoplasma and bacteria. TLR6 collaboration with TLR2 is essential for the recognition and signal transduction of diacylated LPs, such as MALP-2 and FSL-1 [2, 3], as well as heat-killed mycoplasma, indicating that TLR6 and TLR2 play a central role in recognizing mycoplasma .
1. Takeuchi O. et al., 1999. TLR6: A novel member of an expanding toll-like receptor family. Gene. 231(1-2):59-65.
2. Takeuchi O. et al., 2001. Discrimination of bacterial lipoproteins by Toll-like receptor 6. Int Immunol, 13(7):933-40
3. Fujita M. et al., 2003. Involvement of leucine residues at positions 107, 112, and 115 in a leucine-rich repeat motif of human Toll-like receptor 2 in the recognition of diacylated lipoproteins and lipopeptides and Staphylococcus aureus peptidoglycans. J Immunol. 171(7):3675-83.