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HEK 293 cells stably transfected with the HA-tagged human TLR3 gene
3-7 x 10e6 cells
293/hTLR3-HA cells were obtained by stable transfection of HEK293 cells with the pUNO-hTLR3-HA plasmid which expresses the human TLR3 gene fused at the 3'end to the influenza hemaglutinine (HA).
The control cell line for 293/hTLR3-HA cells is 293/null.
Antibiotic resistance: blasticidin
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-freeBack to the top
Shipped on dry iceBack to the top
TLR3 recognizes double-stranded RNA (dsRNA), a molecular pattern associated with viral infection. Stimulation with poly(I:C), a synthetic analog of dsRNA, was shown to induce hyporesponsiveness in TLR3-deficient mice and considerable responsiveness in HEK293 cells expressing TLR3 , suggesting a specific recognition to poly(I:C) by TLR3. TLR3 signals mainly through a MyD88-independent pathway involving the TRIF/TICAM1 adapter protein that leads to the production of IFN-ß and causes dendritic cells to mature .
1. Alexopoulou L. et al., 2001. Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3. Nature, 413(6857):732-8
2. Yamamoto M. et al. 2002. Cutting edge: A novel Toll/Il-1 receptor domain-containing adapter that preferentially activates the IFN-ß promoter in the Toll-like receptor signaling. J Immunol, 169(12):6668-6672
3.Kariko K. et al., 2004. Small interfering RNAs mediate sequence-independent gene suppression and induce immune activation by signaling through toll-like receptor 3.J Immunol. 172(11):6545-9.