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2'3'-c-di-AMP

2'3'-c-di-AMP Unit size Cat. code Docs Qty Price
STING ligand
500 µg
tlrl-nacda23
+-
$225.00

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STING ligand - Cyclic [A(2’,5’)pA(3’,5’)p]

2’3’-c-di-AMP is a synthetic analog of the bacterial second messenger 3’3’-cyclic adenosine monophosphate (c-di-AMP) which differs only by one phosphodiester linkage. c-di-AMP is a potent immunostimulant in mammals. It induces production of type I interferons (IFNs) by directly binding to the endoplasmic reticulum-resident receptor STING (stimulator of interferon genes) [1]. When used at low concentrations in various cellular assays, 2’3’-c-di-AMP induces higher levels of type I IFNs than does c-di-AMP.

 

Reference:

1. Jin L. et al., 2011. MPYS is required for IFN response factor 3 activation and type I IFN production in the response of cultured phagocytes to bacterial second messengers cyclic-di-AMP and cyclic-di-GMP. J Immunol. 187(5):2595-601.

Figures

IRF INDUCTION (Lucia luciferase reporter)
IRF INDUCTION (Lucia luciferase reporter)

THP1-Dual™ cells were stimulated for 24 hours with the STING ligands as shown (all at 10 μg/ml). IRF induction was determined by measuring the relative light units (RLUs) in a luminometer using QUANTI‑Luc™, a Lucia luciferase detection reagent.


THP1-Dual™ cells were stimulated for 24 hours with the STING ligands as shown (all at 10 μg/ml). NF‑kB induction was determined using QUANTI‑Blue™, a SEAP detection reagent, and by reading the optical density (OD) at 655 nm. Non-induced cells (NI) have been included as a negative control.

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Specifications

Description: STING agonist

Synonym: 2’3’-c-di-AMP sodium salt; 2',5'-3',5'-c-diAMP; cyclic (adenosine-(2'→5')-monophosphate-adenosine-(3'→5')-monophosphate); c[A(2',5')pA(3',5')p]

CAS number: 1638241-77-6

Formula: C20H22N10O12P2 .2Na

Molecular weight: 702.38

Purity: ≥ 95% by LC/MS

Solubility: 50 mg/ml in water

Quality control:

  • The biological activity is assessed by measuring induction of the interferon pathway in THP1-Blue™ ISG cells.
  • The absence of endotoxins and other bacterial contaminants has been confirmed using HEK-Blue™ TLR4 and HEK-Blue™ TLR2 cells.
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Contents

  • 500 μg lyophilized c-di-AMP
  • 1.5 ml endotoxin-free water

room temperature Product is shipped at room temperature.

store Product should be stored at -20°C.

Product is sterile filtered prior to lyophilization.

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