2-Aminopurine

PKR Inhibitor

2-aminopurine (2-AP) is a potent inhibitor of double-stranded RNA (dsRNA)-activated protein kinase (PKR), a critical mediator of apoptosis.

PKR is phosphorylated and activated by dsRNA and poly(I:C) [1] and contributes to the induction of type I interferons, such as IFN-β, which can further increase its expression [2].

PKR plays also a role in TLR-induced antiviral activities as an intermediary in TLR3, TLR4 and TLR9 signaling [3].

References

1.Kaufman RJ., 1999. Double-stranded RNA-activated protein kinase mediated virus-induced apoptosis: a new role for an old actor. Proc. Natl. Acad. Sci. USA 96 (21):11693-5.
2. Samuel CE., 2001. Antiviral actions of interferons. Clin Microbiol Rev. 14(4):778-809.
3. García MA. et al., 2006. Impact of Protein Kinase PKR in cell biology: from antiviral to antiproliferative action. Microbiol. Mol. Biol. Rev., 70: 1032 - 1060.

Specifications

Working concentration: 1 - 10 mM

Purity: ≥ 97% UHPLC

CAS number: 452-06-2

Molecular weight: 135.1

Solubility: 20 mg/ml in phosphate-buffered saline:glacial acetic acid (200:1) with heating at 65°C for 15 minutes

Contents

2-Aminopurine is provided as a solid.

• 250 mg 2-aminopurine

2-Aminopurine is shipped at room temperature.

Store at 4 ̊C.

Solid product is stable 1 year at 4°C