THP1-XBlue™-MD2-CD14 Cells

THP1-XBlue™-MD2-CD14 cells derive from the THP1-XBlue™ cell line. THP1-XBlue™-MD2-CD14 cells stably express the human MD2 and CD14 genes. MD2 is an accessory molecule essential for LPS-induced TLR4 response. CD14, a macrophage-specific differentiation antigen, interacts with several TLRs, including TLR4 and TLR2. Overexpression of CD14 was found to increase the response to the majority of TLR ligands.

THP1-XBlue™-MD2-CD14 cells express an NF-κB/AP-1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene. TLR stimulation in THP1-XBlue™-MD2-CD14 cells induces signaling cascades leading to the activation of NF-κB and AP-1 and the subsequent production of SEAP. The reporter protein is easily detectable and measurable when using QUANTI-Blue™, a medium that turns purple/blue in the presence of SEAP.


Figures for this product

TLR and NOD stimulation profile in THP1-XBlue™ and THP1-XBlue™-MD2-CD14


Antibiotic resistance: G418, Zeocin™

Growth medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml)

Guaranteed mycoplasma-free

Shipped on dry ice


1 vial containing 3-7 x 106 cells

100 μl G418 (100 mg/ml)

100 μl Zeocin™ (100 mg/ml)

1 ml Normocin™ (50 mg/ml)

1 pouch of QUANTI-Blue™ (100 ml)

100 μg LPS-EK (E. coli K12 lipopolysaccharide, positive control for TLR4 activity)


Recent articles using InvivoGen THP1-XBlue™-MD2-CD14 Cells


THP1 -XBlue™-MD2-CD14 cells

Description NF-kB/AP-1 Reporter MD2 CD14 co-transfected Monocytes
Cat. Codethpx-mdcdsp
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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