THP1-XBlue™-MD2-CD14 Cells
THP1-XBlue™-MD2-CD14 cells derive from the are derived from the THP1-XBlue™ cell line by cotransfection of the human MD2 and CD14 genes. MD2 is an accessory molecule essential for LPS-induced TLR4 response. CD14, a macrophage-specific differentiation antigen, interacts with several TLRs, including TLR4 and TLR2. Overexpression of CD14 was found to increase the response to the majority of TLR ligands.
THP1-XBlue™-MD2-CD14 cells express an NF-κB/AP-1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene. TLR stimulation in THP1-XBlue™-MD2-CD14 cells induces signaling cascades leading to the activation of NF-κB and AP-1 and the subsequent production of SEAP. The reporter protein is easily detectable and measurable when using QUANTI-Blue™, a medium that turns purple/blue in the presence of SEAP.
Specifications
Antibiotic resistance: G418, Zeocin™
Growth medium: RPMI, 2-4 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Contents
1 vial containing 3-7 x 106 cells
100 μl G418 (100 mg/ml)
100 μl Zeocin™ (100 mg/ml)
1 ml Normocin™ (50 mg/ml)
1 pouch of QUANTI-Blue™ (100 ml)
100 μg LPS-EK (E. coli K12 lipopolysaccharide, positive control for TLR4 activity)
Details
TLR and NOD stimulation profile in THP1-XBlue™ and THP1-XBlue™-MD2-CD14.
Cells were incubated with 1 ng/ml Pam3CSK4 (TLR1/2), 10 ng/ml FSL-1 (TLR2/6), 107 cells/ml HKLM (TLR2), 10 μg/ml poly(I:C) (TLR3), 10 ng/ml LPS-EK (TLR4), 10 ng/ml RecFLA-ST (TLR5), 5 μg/ml imiquimod (TLR7), 5 μg/ml CL075 (TLR8), 10 μg/ml ODN2006 (TLR9), 100 ng/ml C12-iEDAP (NOD1) or 10 μg/ml MDP (NOD2). After 24h incubation, TLR/NOD stimulation was assessed by measuring the levels of SEAP in the supernatant by using QUANTI-Blue™.
ORDERING
THP1 -XBlue™-MD2-CD14 cells
| Description | NF-kB/AP-1 Reporter MD2 CD14 co-transfected Monocytes |
|---|---|
| Cat. Code | thpx-mdcdsp |
| Unit Size | 3-7 x 10e6 |
| Price | For price or distributor address, please select your country |
