THP1-XBlue™-defMyD Cells
THP1-XBlue™-defMyD cells are THP1-XBlue™ cells deficient in MyD88 activity. MyD88 is an adaptor protein involved in many signaling pathways. THP1-XBlue™-defMyD cells are unable to respond to the activation of receptors which signal through MyD88, such as TLR2, TLR4 and IL-1Rs. They remain responsive to MyD88-independent receptors, such as NOD1 and TNFR.
THP1-XBlue™-defMyD cells express an NF-κB/AP-1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene. Activation of MyD88-independent signaling pathways in THP1-XBlue™-defMyD cells triggers the activation of NF-κB and AP-1 and the subsequent production of SEAP. The reporter protein is easily detectable and measurable when using QUANTI-Blue™, a medium that turns purple/blue in the presence of SEAP.
Specifications
Antibiotic resistance: Hygromycin B, Zeocin™
Growth medium: RPMI, 2-4 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Contents
1 vial containing 3-7 x 106 cells
100 μl HygroGold™ (ultra-pure hygromycin B; 100 mg/ml)
100 μl Zeocin™ (100 mg/ml)
1 ml Normocin™ (50 mg/ml)
1 pouch of QUANTI-Blue™ (100 ml)
25 μg Tri-DAP (positive control for NOD1 activity)
Details
MyD88-dependent and -independent responses in THP1-XBlue™ and THP1-XBlue™-defMyD.
Cells were incubated with 1 μg/ml FSL-1 (TLR2/6), 1 μg/ml LPS-EK (TLR4), 5 μg/ml CL075 (TLR8), 10 μg/ml Tri-DAP (NOD1) and 50 ng/ml TNF-α. After 24h incubation, NF-κB activation was determined using QUANTI-Blue™.
ORDERING
THP1-XBlue™-defMyD cells
| Description | NF-kB/AP-1 Reporter MyD88-deficient Monocytes |
|---|---|
| Cat. Code | thpx-dmyd |
| Unit Size | 3-7 x 10e6 |
| Price | For price or distributor address, please select your country |
