THP1-XBlue™-CD14 Cells
THP1-XBlue™-CD14 cells derive from the THP1-XBlue™ cell line by stable transfection of the human CD14 gene, encoding a macrophage-specific differentiation antigen. CD14 interacts with several TLRs in the induction of the signaling cascade upon TLR stimulation and its overexpression was found to increase the reponse to the majority of TLR ligands.
THP1-XBlue™-CD14 cells express an NF-κB/AP-1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene. TLR stimulation in THP1-XBlue™-CD14 cells induces signaling cascades leading to the activation of NF-κB and AP-1 and the subsequent production of SEAP. The reporter protein is easily detectable and measurable when using QUANTI-Blue™, a medium that turns purple/blue in the presence of SEAP.
Specifications
Antibiotic resistance: G418, Zeocin™
Growth medium: RPMI, 2-4 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Contents
1 vial containing 3-7 x 106 cells
100 μl G418 (100 mg/ml)
100 μl Zeocin™ (100 mg/ml)
1 ml Normocin™ (50 mg/ml)
1 pouch of QUANTI-Blue™ (100 ml)
100 μg LPS-EK (E. coli K12 lipopolysaccharide, positive control for TLR4 activity)
ORDERING
THP1 -XBlue™-CD14 cells
| Description | NF-kB/AP-1 Reporter CD14 transfected Monocytes |
|---|---|
| Cat. Code | thpx-cd14sp |
| Unit Size | 3-7 x 10e6 |
| Price | For price or distributor address, please select your country |
