THP1-XBlue cells

NF-κB/AP-1 Reporter Monocytes
THP1-XBlue™ cells are derived from THP-1, a human monocytic cell line that naturally expresses many pattern-recognition receptors, including Toll-like receptors. They stably express an NF-κB/AP-1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter system to facilitate the monitoring of TLR-induced NF-κB/AP-1 activation.
Four cell lines are available: THP1-XBlue™, THP1-XBlue™-CD14, which overexpresses the cell surface protein CD14 for enhanced sensitivity, THP1-XBlue™-MD2-CD14, which overexpresses MD2 and CD14 to increase the response toTLR ligands and THP1-XBlue™-defMyD characterized by a deficient MyD88 activity.
