THP1-Dual™ KI-hSTING-S154 CellsNEW

THP1-DualKI-hSTING-S154 cells were generated from THP1-Dual™ KO-STING cells by knockin of the intronless coding sequence (from the ATG to the TGA) of the S154 hSTING variant. The allele S154 contains a unique point mutation (N154S) in the “wild-type” R232-RGR hSTING variant. This mutation confers gain-of-function and constitutive activation of STING with an upregulation of IFN production. It is associated with a chronic autoinflammatory disease, known as STING-associated vasculopathy with onset in infancy (SAVI) [3].

THP1‑Dual™ KI-hSTING-S154 cells are resistant to blasticidin and Zeocin™.

Figures for this product

Evaluation of IRF- and NF-κB-induced responsesEvaluation of inhibitors on constitutive IRF-induced response


Antibiotic resistance: Zeocin™blasticidin

Growth medium:  RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml)

Quality control
• The biallelic knockin (KI) of the human STING variant (S154) has been verified by functional assays, PCR and sequencing.
• Reporter activity has been validated by stimulating the cells with STING ligands, such as c-di-AMP, cGAMP.
• The cell line stability for 20 passages following thawing has been verified.
• The cell line is guaranteed mycoplasma-free.

Shipped on dry ice

This product is covered by a Limited Use License (See Terms and Conditions).


• 1 vial of THP1-Dual™ KI-hSTING-S154 cells (3-7 x 106 cells) in freezing medium.
• 1 ml Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi. 
• 100 μl Zeocin™ (100 mg/ml).
• 100 μl Blasticidin (10 mg/ml).
• 1 pouch of QUANTI-Luc™ (Lucia luciferase detection medium).
• 1 pouch of QUANTI-Blue™ (SEAP detection medium).

IMPORTANT: Cells are shipped frozen. If cells are not frozen upon arrival, contact InvivoGen immediately.



STING (stimulator of interferon genes) is essential for the interferon (IFN) response to cytoplasmic foreign or self-DNA. It directly senses cyclic dinucleotides (CDNs), which are important messengers in bacteria and innate immune agonists in mammals [1]. Distinct variants of human STING (hSTING) that affect CDN recognition and signal transduction have been identified: 

• R232 (R71‑G230-R232-R293): the most prevalent in the human population (~60%). Referred as the “wild-type” or 232R-RGR allele [2].

• HAQ (H71A230-R232-Q293): contains three non-synonymous single nucleotide substitutions; R71H, G230A and R293Q. This allele, found in ~20% of the population, is less sensitive to CDNs than the “wild-type” allele [2].

• H232 (R71‑G230-H232-R293): the most commonly used hSTING variant in structural studies [2].

S154: a gain-of-function mutation resulting in constitutive STING activation [3].

• M155: a gain-of-function mutation resulting in constitutive STING activation [3,4].

• A162: a synthetic mutation (S162A) that confers hSTING sensitivity to DMXAA, a tumor vascular disrupting agent in mice [5].

THP1-DualKI-STING cells are a family of reporter cells allowing the study of STING variation by monitoring the activation of the transcription factors ISRE (IFN-stimulated response elements) and NF-kB. They were generated from THP1-DualKO-STING cells, which derive from the human THP-1 monocytes, by stable biallelic knockout of the endogenous HAQ hSTING gene and stable integration of two inducible secreted reporter genes: Lucia luciferase and SEAP (secreted embryonic alkaline phosphatase). The Lucia luciferase gene is under the control of an ISG54 (interferon-stimulated gene) minimal promoter in conjunction with five ISRE. The SEAP gene is driven by an IFN-β minimal promoter fused to five copies of the NF-kB response element. As a result, they allow the simultaneous study of the IFN regulatory factor (IRF) and NF-kB pathways. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Luc, a Luciadetection reagent, and QUANTI-Blue, a SEAP detection reagent.


1. Sun L. et al., 2013. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science. 339:786-91.
2. Yi G. et al., 2013. Single nucleotide polymorphisms of human STING can affect innate immune response to cyclic dinucleotides. PLOS One. 8:e77846.
3. Liu Y. et al., 2014. Activated STING in a vascular and pulmonary syndrome. N Engl J Med. 371:507-18.
4. Jeremiah N. et al., 2013. Inherited STING-activating mutation underlies a familial inflammatory syndrome with lupus-like manifestations. J Clin Invest. 124:5516-20.
5. Gao P. et al., 2014. Binding-pocket and lid-region substitutions render human STING sensitive to the species-specific drug DMXAA. Cell Reports. 8:1668-76.


THP1-Dual™ KI-hSTING-S154 CellsNEW!

Description STING (S154 isoform; constitutively active) knockin NF-kB-SEAP and IRF-Lucia Reporter Monocytes
Cat. Codethpd-s154
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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