Our Products
CpG-free DNA
Bacterial DNA is rich in unmethylated 2’-deoxyribo (cytidine-phosphateguanosine) (CpG) dinucleotides, in contrast to mammalian DNA which contains a low frequency of CpG dinucleotides that are mostly methylated. Unmethylated CpGs in specific sequence contexts activate the vertebrate immune system. Genes with these sequences are recognized as foreign DNA by the vertebrate host leading to a progressive decline of their expression.
CpG-free Cloning Vectors - pCpG Plasmids
InvivoGen has developed a new family of plasmids that are completely devoid of CpG dinucleotides, named pCpG. These plasmids yield high levels of transgene expression both in vitro and in vivo, and allow sustained expression in vivo. InvivoGen provides 4 families of pCpG plasmids: pCpGfree for in vivo studies, pCpGfree-vitro for in vitro and in vivo applications, pCpGfree-siRNA for long term production of siRNAs in vivo and pCpGfree-siRNADUO for long term production of two siRNAs simultaneously in vivo.
Many non-mammalian genes are widely used as resistance, reporter or cytotoxic genes in molecular and cellular studies. InvivoGen has synthesized new alleles of these genes, with reduced or no CpG dinucleotides. These synthetic genes display higher activity and lower immunogenicity than their wild-type counterparts. Three gene families are available in pMOD: Reporter Genes, Resistance Genes and Suicide (cytotoxic) Genes.
InvivoGen is an expert in developing CpG-Free genes and now offers a CpG-Free gene custom service. Send us the sequence of your gene of interest, and our specialists will design, synthesize and clone into an expression vector a CpG-Free allele of this gene. We guarantee that the sequence of the synthetic gene will 100% match the designed sequence.