Our Products

TLR Cell Lines

InvivoGen provides researchers with the most comprehensive set of TLRs expressing and TLR reporter cells. These cells enable efficient monitoring of TLR activity using ELISA analysis such as IL-8 titration or reporter-based systems that monitor TLR-induced NF-κB activation.

Check Reporter Gene activation of TLRs, NODs, RIG-I/ MDA-5 in InvivoGen's Reporter Cell lines

HEK293/TLR cells

293/TLR clones iconCells that constitutively express a given functional TLR gene are valuable tools for many applications, such as the study of the mechanisms involved in TLR recognition or signaling, and the development of new potential therapeutic drugs. InvivoGen provides HEK293 cells stably transfected with a plasmid that constitutively expresses one or two TLR and/or TLR-related genes, of human or mouse origin.

HEK293/TLR-HA cells

293/TLR-HA clones iconHEK293/hTLR-HA clones were generated to facilitate the detection of TLR expression by Western Blot through the HA tag. HEK293 cells were stably transfected with plasmids expressing the human TLR genes fused to the HA tag (3‘ end of the influenza hemaglutinine). This tag is the epitope of a potent and specific monoclonal antibody.

HEK-Blue™ TLR cells NEW

HEK-Blue TLR cells iconInvivoGen introduces HEK-Blue™ TLR cells, a collection of HEK293 TLR reporter cells that are engineered to provide a rapid, sensitive and reliable method to screen and validate TLR agonists or antagonists. HEK-Blue cells express an NF-κB-inducible SEAP reporter gene.

THP1-XBlue™ cells

THP1-Blue clones iconTHP1-XBlue™ Clones are derived from THP-1, a human immune cell line that naturally expresses most TLRs transfected. They stably express an NF-κB-inducible reporter (SEAP) system to facilitate the monitoring of TLR-induced NF-κB activation. 3 clones are available: THP1-XBlue™, THP1-XBlue™-CD14 and THP1-XBlue™-defMyD.

Ramos-Blue™ cells - NF-κB/Ap-1 reporter B lymphocytes

Ramos-Blue cells iconRamos-Blue™ are B lymphocyte cell lines that stably expresses an NF-κB/AP-1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene. Ramos-Blue™ cells can be used to study  the TLR3, TLR7, TLR9 and NOD1 signaling pathways. 

RAW-Blue™ cells - Mouse Macrophage Reporter Cells

Raw-Blue cells iconMacrophages are major players in the innate immune defense. They express a large repertoire of different classes of pattern recognition receptors (PRRs), such as the TLRs, RLRs and NLRs. RAW-Blue™ Cells are murine macrophages designed for the study of these PRRs. They stably express an NF-κB-inducible SEAP reporter gene and in combination with QUANTI-Blue™, a SEAP detection medium, provide a rapid and powerful method to monitor the activation of these PRRs.

TLR4 Reporter cells

TLR4 reporter cells iconTLR4 is extensively studied as it is the major receptor involved in the recognition of lipopolysaccharide (LPS) responsible for sceptic shock. InvivoGen provides three cell lines for TLR4 studies: C3H/TLR4mut and C3H/WT murine embryonic fibroblasts (MEFs), and HEK-Blue™hTLR4 Cells.

Reporter Gene activation of TLRs and NODs in InvivoGen's TLR Reporter cell lines

Reporter cell line   Reporter system TLR1 TLR2 TLR3 TLR4 TLR5 TLR6 TLR7 TLR8 TLR9 NOD1 NOD2
HEK-Blue™ hTLR2 human NF-κB/AP-1 SEAP E ++ E - E E - - - E -
HEK-Blue™ hTLR3 human NF-κB/AP-1 SEAP E - ++ - E E - - - E -
HEK-Blue™ hTLR4 human NF-κB/AP-1 SEAP E - E ++ E E - - - E -
HEK-Blue™ hTLR5 human NF-κB/AP-1 SEAP E - E - ++ E - - - E -
HEK-Blue™ hTLR7 human NF-κB/AP-1 SEAP E - E - E E ++ - - E -
HEK-Blue™ hTLR8 human NF-κB/AP-1 SEAP E - E - E E - ++ - E -
HEK-Blue™ hTLR9 human NF-κB/AP-1 SEAP E - E - E E - - ++ E -
HEK-Blue™ hNOD1 human NF-κB/AP-1 SEAP E - E - E E - - - ++ -
HEK-Blue™ hNOD2 human NF-κB/AP-1 SEAP E - E - E E - - - E ++
HEK-Blue™ Null1 human NF-κB/AP-1 SEAP E - E - E E - - - E -
HEK-Blue™ Null2 human NF-κB/AP-1 SEAP E - E - E E - - - E -

E: endogenous expression,  ++: overexpression, - : no expression

Reporter Gene activation of TLRs, NODs, RIG-I/ MDA-5 in InvivoGen's Reporter Cell lines

Reporter cell lines   Reporter system TLR1 TLR2 TLR3 TLR4 TLR5 TLR6 TLR7 TLR8 TLR9 NOD1 NOD2 RIG-I/MDA-5
THP1-XBlue human NF-κB/AP-1 SEAP ++ ++ -
 +
 +/- ++ - ++
 - + +
 ND
THP1-XBlue-CD14 human NF-κB/AP-1 SEAP ++ ++ - + +/- ++ - ++ - + +
 ND
THP1-XBlue-defMyD  human NF-κB/AP-1 SEAP -
-
-
-
-
-
 -
-
-
+
+
ND
Ramos-Blue human NF-κB/AP-1 SEAP +/- +/- ++ - - +/- ++ - + + - ND
Ramos-Blue-defMyD human NF-κB/AP-1 SEAP - - ++ - - +/- - - - + - ND
RAW-Blue murine NF-κB/AP-1 SEAP ++ ++ +/- ++ - ++ + ND + +/- + ND
MEFs C3H WT
 murine NF-κB/AP-1 SEAP + + +/- + +/- + - ND - ND ND +
MEFs C3H /TLR4 mut
 murine NF-κB/AP-1 SEAP + + +/- - +/- + - ND - ND ND +
MEFs C57
 murine NF-κB/ISRE SEAP +
 +
 -
 +
 +/-
 +
 - ND
 -
 +
 - +
B16-Blue IFN-α/β murine ISRE SEAP - - +/- - - - - - - - - ++

++: strongly induced, +: induced, +/-: lightly induced, -: non induced, ND: non described

Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.

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