Open Reading Frames - Gene A-List™ - Human and Murine ORFs
Gene A-List™ is a collection of high quality, full-length sequenced human and mouse open reading frames (ORFs) available in an expression vector ready-to-use for protein expression.
All ORFs are cloned downstream of a strong and ubiquitous mammalian promoter which makes these clones suitable for expression and functional studies in various mammalian cell lines.
Features and Benefits
Full-length Sequences Open Reading Frames
• Natives genes
• Fusion genes
InvivoGen provides a large collection of human and murine genes, fully sequenced. These genes are provided as open reading frames (ORFs) from the ATG to the Stop codon, excluding introns and untranslated regions.
As the ORFs are amplified from cDNA banks, a variant form is sometimes obtained. Most of the variations have been reported in Genbank. Furthermore for cloning convenience, a neutral amino acid is sometimes added immediately after the Start codon in order to create a suitable cloning site.
Among the Gene A-List™, some genes encode proteins that are naturally secreted. Their sequence include their native signal sequence which is generally located at the 5' end of the coding sequence. Other genes that code for engineered proteins, such as particular fragments of longer proteins (i.e. angiostatic proteins) may include the signal sequence of the human IL-2 gene between the ATG and the second codon to allow their secretion.
Suitable for Expression in Mammalian Cells
Each ORF is cloned in a mammalian expression cassette consisting of a potent and ubiquitous composite promoter and the strong SV40 polyadenylation signal.
Each ORF is flanked by a unique restriction site at the 5’ end and 3’ end to facilitate its subcloning into another vector.
The ORFs are provided in a mammalian expression plasmid. Several plasmid backbones are used:
- pORF : a simple plasmid selectable only in E. coli with ampicillin. pORF is also suitable for expression in mammalian cells
- pBLAST : a plasmid selectable in mammalian cells and in E. coli with blasticidin.
- pUNO : a plasmid selectable in mammalian cells and in E. coli with blasticidin.
- pDUO : a plasmid containing two genes selectable in mammalian cells and in E. coli with blasticidin. Some are selectable with hygromycin (pDUO2).
- pZERO : a plasmid selectable in mammalian cells and in E. coli with puromycin.
- pMOD : a simple plasmid selectable only in E. coli with ampicillin