Our Products
- LacZ Detection Kits
- Alkaline phosphatase Detection
Reporter Detection Kits
Gene reporter systems are extensively used for the study of eukaryotic gene expression and regulation. Although they play a significant role in numerous applications, they most frequently serve as indicators of transcriptional activity in cells.
The reporter gene of choice is combined to a promoter sequence and can be subsequently assayed in vitro or in vivo. An ideal reporter gene is not endogenously expressed by the target cells creating background signals and can be detected through assays that are easy, sensitive and reliable.
InvivoGen provides two reporter gene systems:
β-Galactosidase (LacZ) reporter gene system
The LacZ Detection Kits provide simple and convenient methods for the visual detection of LacZ expression within cells or tissues.
Alkaline phosphatase (AP) reporter gene system
Secreted alkaline phosphatase (SEAP) is extremely heat stable and resistant to inhibitor. SEAP is secreted into cell culture supernatant and therefore offers many advantages over intracellular reporters :
- Determine reporter activity without disturbing cells
- Can be used for kinetics studies
- No need to prepare cell lysates
- Short hands-on-time
InvivoGen offers three different methods to determine SEAP activity:
SEAP Reporter Assay Kit - Detection kit for the quantification of SEAP
QUANTI-Blue™ - Detection medium for the detection and quantification of any AP
HEK-Blue™ Detection - Cell culture medium for real-time, one step detection of SEAP