Our Products
Ready-made psiRNA
Plasmids Expressing Functional shRNAs
Ready-made psiRNA is a new family of plasmids expressing a growing list of shRNAs which functionality has been described in the literature or validated in house.
Ready-made psiRNA plasmids eliminate the need to design and clone several siRNA sequences before identifying an effective one.
They express shRNAs that silence the expression of a target gene by >70%.
Major Gene Families Targeted by Ready-Made psiRNAs :
- Autophagy-related shRNAs
- Remodeling shRNAs
- Disease-related shRNAs
- Signaling effectors shRNAs
- Reporter shRNAs
- Inhibitor of Signaling shRNA
- Cytokine & Related shRNAs
- TLR shRNAs
- TLR-Related shRNAs
- NLR shRNAs
- RLR shRNAs
- Pathogen Sensors shRNAs
- DAMPs-Related shRNAs
- Miscellaneous shRNAs
- All InvivoGen‘s ORFs
Features and Benefits
Ready-made psiRNAs are psiRNA-h7SKGFPzeo-derived plasmids. They feature the human 7SK RNA Pol III promoter that generates high amounts of short hairpin RNAs.
They also feature the GFP::Zeo fusion gene which confers both reporter and antibiotic resistance activities making these plasmids very useful for the following applications:
• Monitor transfection efficiency
• Standardize gene silencing efficiency
• Select clones that stably express a validated siRNA
The silencing efficiency of each Ready-made psiRNA plasmid has been tested using the psiTEST system.
Silence All Your Genes of Interest with the Same siRNA
Most of InvivoGen’s plasmids carry the late SV40 polyadenylation signal (polyA). A siRNA has been designed against this polyA and cloned into psiRNAh7SKGFPzeo.
This unique Ready-made psiRNA has been verified experimentally to reduce the expression by >70% of a variety of genes cloned upstream of this polyA. Thus with a single psiRNA you will be able to silence an array of genes cloned in one of InvivoGen’s plasmids.
Contents and Storage
Ready-made psiRNA plasmids are available alone or in a kit.
Each Ready-made psiRNA plasmid is provided as 20 µg of lyophilized DNA.
Each Ready-made psiRNA kit contains the following components:
- 20 µg of a Ready-made psiRNA plasmid
- 20 µg of a control psiRNA plasmid (psiRNA-Luc)
- 1 vial of LyoComp GT116
- 4 pouches of Fast-Media® Zeo
Products are shipped at room temperature. Store at -20°C.