Design hairpin insert

This tool generates the sequence of two complementary oligonucleotides necessary to create the hairpin insert for psiRNA cloning vectors.


1. Enter the siRNA hairpin name for reference (optional)
2. Enter the sense siRNA target sequence. (Spaces in the sequence will be ignored)
3. Enter the desired loop sequence. (The default sequence is the one used by InvivoGen for psiRNA vectors).
4. Select your psiRNA cloning vector.
Our vectors offer two cloning options : Bbs I / Bbs I and Acc 65I / Hind III.

siRNA name :

siRNA target sequence :
5' 3'

Loop sequence :
psiRNA cloning vector:

Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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