Research Areas
- Innate Immunity
- Toll-Like Receptors
- RIG-I-Like Receptors
- Nod-Like Receptors
- Autophagy and TLRs
- Other Pathogen Sensors
- Inflammasomes
- Danger Signals
- Inhibitors
- Blues™ Reporter Cells
- Lentivirus Production
- Cytokine Signaling
- Immunoglobulin A
- RNA Interference
- Gene Therapy
- Cancer Research
- Cell Culture & Transfection
- Expression Vectors
Literature
Publication Highlights
· Judge, AD. et al., 2005. "Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA." Nat Biotechnol 23(4): 457-62 [PDF]
· Loiarro, M. et al., 2005. "Peptide-mediated interference of TIR domain dimerization in MyD88 inhibits interleukin-1-dependent activation of NFkB." J Biol Chem 280(16): 15809-14. [PDF]
· Kandimalla, ER. et al., 2005. "Immunomodulatory oligonucleotides containing a cytosine-phosphate- 2'-deoxy-7-deazaguanosine motif as potent toll-like receptor 9 agonists." Proc Natl Acad Sci U S A 102(19): 6925-30. [PDF]
· Raffatellu M. et al., 2005. "The Vi capsular antigen of Salmonella enterica serotype Typhi reduces Toll-like receptor-dependent interleukin-8 expression in the intestinal mucosa." Infect Immun. 73(6):3367-74 [PDF]
Mycoplasma eradication & prevention with Plasmocin
· Julie Guillermet-Guibert, et al. (2009) Targeting the sphingolipid metabolism to defeat pancreatic cancer cell resistance to the chemotherapeutic gemcitabine drug. Mol. Cancer Ther., Apr 2009; 8: 809 - 820.
· Anna Vilborg, Jacob A. Glahder, Margareta T. Wilhelm, Cinzia Bersani, Martin Corcoran, Salah Mahmoudi, Maiken Rosenstierne, Dan Grandér, Marianne Farnebo, Bodil Norrild, and Klas G. Wiman (2009) The p53 target Wig-1 regulates p53 mRNA stability through an AU-rich element. PNAS, Sep 2009; 106: 15756 - 15761.
· Lucie JETTÉ, et al. (2008) Resistance of Colorectal Cancer Cells to 5-FUdR and 5-FU Caused by Mycoplasma Infection. Anticancer Res, Jul 2008; 28: 2175 - 2180.
· Alina I. Marusina, Steven J. Burgess, Ishani Pathmanathan, Francisco Borrego, and John E. Coligan (2008) Regulation of Human DAP10 Gene Expression in NK and T Cells by Ap-1 Transcription Factors. J. Immunol., Jan 2008; 180: 409 - 417.
· Stephanie L. Bissonnette, Jessica E. Teague, David H. Sherr, and Jennifer J. Schlezinger (2008) An Endogenous Prostaglandin Enhances Environmental Phthalate-Induced Apoptosis in Bone Marrow B Cells: Activation of Distinct but Overlapping Pathways. J. Immunol., Aug 2008; 181: 1728 - 1736.
· Jennifer J. Schlezinger, Jessica K. Emberley, Stephanie L. Bissonnette, and David H. Sherr (2007) An L-Tyrosine Derivative and PPAR Agonist, GW7845, Activates a Multifaceted Caspase Cascade in Bone Marrow B Cells. Toxicol. Sci., Jul 2007; 98: 125 - 136.
· Sanae Uchida, et al. (2006) Amino Acids C-Terminal to the 14-3-3 Binding Motif in CDC25B Affect the Efficiency of 14-3-3 Binding. J. Biochem., Apr 2006; 139: 761 - 769.
·Michael E. Rusiniak, Min Yu, Douglas T. Ross, Erin C. Tolhurst, and James L. Slack (2000) Identification of B94 (TNFAIP2) as a Potential Retinoic Acid Target Gene in Acute Promyelocytic Leukemia. Cancer Res., Apr 2000; 60: 1824 - 1829.
pFUSE-Fc vectors
· Terry K. Means, Eleftherios Mylonakis, Emmanouil Tampakakis, Richard A. Colvin, Edward Seung, Lindsay Puckett, Melissa F. Tai, Cameron R. Stewart, Read Pukkila-Worley, Suzanne E. Hickman, Kathryn J. Moore, Stephen B. Calderwood, Nir Hacohen, Andrew D. Luster, and Joseph El Khoury (2009) Evolutionarily conserved recognition and innate immunity to fungal pathogens by the scavenger receptors SCARF1 and CD36. J. Exp. Med., Mar 2009; 206: 637 - 653.
· Qi-Quan Huang, Rudina Sobkoviak, Angela R. Jockheck-Clark, Bo Shi, Arthur M. Mandelin, II, Paul Peter Tak, G. Kennith Haines, III, Christopher V. Nicchitta, and Richard M. Pope (2009) Heat Shock Protein 96 Is Elevated in Rheumatoid Arthritis and Activates Macrophages Primarily via TLR2 Signaling. J. Immunol., Apr 2009; 182: 4965 - 4973.
· Tetsuyuki Takahashi, Hisanori Uehara, Yoshimi Bando, and Keisuke Izumi (2008) Soluble EP2 neutralizes prostaglandin E2-induced cell signaling and inhibits osteolytic tumor growth. Mol. Cancer Ther., Sep 2008; 7: 2807 - 2816.
· Tomohisa Baba, Sari Iwasaki, Takako Maruoka, Akira Suzuki, Utano Tomaru, Hitoshi Ikeda, Takashi Yoshiki, Masanori Kasahara, and Akihiro Ishizu (2008) Rat CD4+CD8+ Macrophages Kill Tumor Cells through an NKG2D- and Granzyme/Perforin-Dependent Mechanism. J. Immunol., Mar 2008; 180: 2999 - 3006.
· Katsue Suzuki-Inoue, Yukinari Kato, Osamu Inoue, Mika Kato Kaneko, Kazuhiko Mishima, Yutaka Yatomi, Yasuo Yamazaki, Hisashi Narimatsu, and Yukio Ozaki (2007) Involvement of the Snake Toxin Receptor CLEC-2, in Podoplanin-mediated Platelet Activation, by Cancer Cells. J. Biol. Chem., Sep 2007; 282: 25993 - 26001.
TLR-ligands
· Sanna M. Mäkelä, Mari Strengell, Taija E. Pietilä, Pamela Österlund, and Ilkka Julkunen (2009) Multiple signaling pathways contribute to synergistic TLR ligand-dependent cytokine gene expression in human monocyte-derived macrophages and dendritic cells. J. Leukoc. Biol., Apr 2009; 85: 664 - 672.
· Jooeun Lee, Ivan Tattoli, Kacper A. Wojtal, Stephan R. Vavricka, Dana J. Philpott, and Stephen E. Girardin (2009) pH-dependent Internalization of Muramyl Peptides from Early Endosomes Enables Nod1 and Nod2 Signaling. J. Biol. Chem., Aug 2009; 284: 23818 - 23829.
· Ute Koch, Emma Fiorini, Rui Benedito, Valerie Besseyrias, Karin Schuster-Gossler, Michel Pierres, Nancy R. Manley, Antonio Duarte, H. Robson MacDonald, and Freddy Radtke (2008) Delta-like 4 is the essential, nonredundant ligand for Notch1 during thymic T cell lineage commitment. J. Exp. Med., Oct 2008; 205: 2515 - 2523.
· Nicole L. Yonkers, Benigno Rodriguez, Kimberly A. Milkovich, Robert Asaad, Michael M. Lederman, Peter S. Heeger, and Donald D. Anthony (2007) TLR Ligand-Dependent Activation of Naive CD4 T Cells by Plasmacytoid Dendritic Cells Is Impaired in Hepatitis C Virus Infection. J. Immunol., Apr 2007; 178: 4436 - 4444.
· Elise Chiffoleau, Jean-Marie Heslan, Michele Heslan, Cedric Louvet, Thomas Condamine, and Maria-Cristina Cuturi (2007) TLR9 ligand enhances proliferation of rat CD4+ T cell and modulates suppressive activity mediated by CD4+ CD25+ T cell. Int. Immunol., Feb 2007; 19: 193 - 201.
· Jennifer M. Burke, Lisa M. Ganley-Leal, Asma Khatri, and Lee M. Wetzler (2007) Neisseria meningitidis PorB, a TLR2 Ligand, Induces an Antigen-Specific Eosinophil Recall Response: Potential Adjuvant for Helminth Vaccines? J. Immunol., Sep 2007; 179: 3222 - 3230.
· Ulf Yrlid, Simon W. F. Milling, Joanna L. Miller, Sian Cartland, Christopher D. Jenkins, and G. Gordon MacPherson (2006) Regulation of Intestinal Dendritic Cell Migration and Activation by Plasmacytoid Dendritic Cells, TNF- and Type 1 IFNs after Feeding a TLR7/8 Ligand. J. Immunol., May 2006; 176: 5205 - 5212.
TLR-related genes in pUNO
· Antoine Tanne, et al. (2009) A murine DC-SIGN homologue contributes to early host defense against Mycobacterium tuberculosis. J. Exp. Med., Sep 2009; 206: 2205 - 2220.
· Ayaluru Murali, et al. (2008) Structure and Function of LGP2, a DEX(D/H) Helicase That Regulates the Innate Immunity Response. J. Biol. Chem., Jun 2008; 283: 15825 - 15833.
· Han Geun Kim, et al. (2008) Lipoteichoic Acid Isolated from Lactobacillus plantarum Inhibits Lipopolysaccharide-Induced TNF- Production in THP-1 Cells and Endotoxin Shock in Mice. J. Immunol., Feb 2008; 180: 2553 - 2561.
· Ni Cheng, Rong He, Jun Tian, Patrick P. Ye, and Richard D. Ye (2008) Cutting Edge: TLR2 Is a Functional Receptor for Acute-Phase Serum Amyloid A. J. Immunol., Jul 2008; 181: 22 - 26.
· Zhijun Wang, Li Xiang, Junjie Shao, and Zhenghong Yuan (2006) The 3' CCACCA Sequence of tRNAAla(UGC) Is the Motif That Is Important in Inducing Th1-Like Immune Response, and This Motif Can Be Recognized by Toll-Like Receptor 3. Clin. Vaccine Immunol., Jul 2006; 13: 733 - 739.
· Michael G. Kelly, et al. (2006) TLR-4 Signaling Promotes Tumor Growth and Paclitaxel Chemoresistance in Ovarian Cancer. Cancer Res., Apr 2006; 66: 3859 - 3868.
· Jessica K. Bell, Janine Askins, Pamela R. Hall, David R. Davies, and David M. Segal (2006) The dsRNA binding site of human Toll-like receptor 3. PNAS, Jun 2006; 103: 8792 - 8797.
Genes in pORF
· Andrew G. Sikora, et al. (2009) IFNA Enhances Peptide Vaccine-Induced CD8+ T Cell Numbers, Effector Function, and Antitumor Activity. J. Immunol., Jun 2009; 182: 7398 - 7407.
· R. van der Geize, et al. (2008) A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res., Dec 2008; 36: e151.
· Yanyan Lou, Chengwen Liu, Grace J. Kim, Yong-Jun Liu, Patrick Hwu, and Gang Wang (2007) Plasmacytoid Dendritic Cells Synergize with Myeloid Dendritic Cells in the Induction of Antigen-Specific Antitumor Immune Responses. J. Immunol., Feb 2007; 178: 1534 - 1541.
· Wei Lu, Qing Sun, Jin Wan, Zhenjue She, and Xin-Guo Jiang (2006) Cationic Albumin–Conjugated Pegylated Nanoparticles Allow Gene Delivery into Brain Tumors via Intravenous Administration. Cancer Res., Dec 2006; 66: 11878 - 11887.
· Ayako Hino, Satoshi Fukuyama, Kosuke Kataoka, Mi-Na Kweon, Kohtaro Fujihashi, and Hiroshi Kiyono (2005) Nasal IL-12p70 DNA Prevents and Treats Intestinal Allergic Diarrhea. J. Immunol., Jun 2005; 174: 7423 - 7432.
· Kosuke Kataoka, Jerry R. McGhee, Ryoki Kobayashi, Keiko Fujihashi, Satoshi Shizukuishi, and Kohtaro Fujihashi (2004) Nasal Flt3 Ligand cDNA Elicits CD11c+CD8+ Dendritic Cells for Enhanced Mucosal Immunity. J. Immunol., Mar 2004; 172: 3612 - 3619.
· Afzal A. Siddiqui, et al. (2003) Induction of Protective Immunity against Schistosoma mansoni via DNA Priming and Boosting with the Large Subunit of Calpain (Sm-p80): Adjuvant Effects of Granulocyte-Macrophage Colony-Stimulating Factor and Interleukin-4. Infect. Immun., Jul 2003; 71: 3844 - 3851.
pZERO-mTLR4
· Frost, R.A., G.J. Nystrom et al. (2004) Lipopolysaccharides stimulates nitric oxide synthase-2 expression in murine skeletal muscle and C(2)C(12) myoblasts via Toll-like receptor-4 and c-Jun NH(2)-terminal kinase pathways. Am J Physiol Cell Physiol 287(6): c1605-c1615. [PDF]
pZERO-hTLR3
· Guillot, L., R. Le Goffic, et al. (2005). Involvement of toll-like receptor 3 in the immune response of lung epithelial cells to double-stranded RNA and influenza A virus. J Biol Chem 280(7): 5571-80.
pBROAD 3
· Oliver, E.R., T.L. Saunders, et al. (2004). Ribosomal protein L24 defect in belly spot and tail (Bst), a mouse Minute. Development 131(16): 3907-20. [PDF]
pBROAD
· Wang, Y., A.T. Boeck, et al. (2004). Resistance to organophosphorus agent toxicity in transgenic mice expressing the G117H mutant of human butyrylcholinesterase. Toxicol Appl Pharmacol. 196: 356-66.
· Schaefer L. et al. 2005. The matrix component biglycan is proinflammatory and signal through Toll-like receptors 4 and 2 in macrophages. J. Clin Invest 115(8):2223-33. [PDF]
· Baratin M. et al., 2005. Natural killer cell and macrophage cooperation in MyD88-dependent innate responses to Plasmodium falciparum. Proc Natl Acad Sci U S A.102(41):14747-52.
psiRNA-hH1
· Andersson MG. et al., 2005. Suppression of RNA interference by adenovirus virus-associated RNA. J Virol. 79(15):9556-65. [PDF]
· Cao L. et al., 2004. VEGF links hippocampal activity with neurogenesis, learning and memory. Nat Genet. 36(8):827-35.
· Choi WS. et al., 2004. Activation of RBL-2H3 mast cells is dependent on tyrosine phosphorylation of phospholipase D2 by Fyn and Fgr. Mol Cell Biol. 24(16):6980-92. [PDF]
· Fechir M. et al., 2005. Tristetraprolin regulates the expression of the human inducible nitric-oxide synthase gene. Mol Pharmacol. 67(6):2148-61.
· Hwang SG. et al., 2005. Regulation of beta-catenin signaling and maintenance of chondrocyte differentiation by ubiquitin-independent proteasomal degradation of alpha-catenin. J Biol Chem. 280(13):12758-65.
· Kim DJ. et al., 2006. Interaction of SPIN90 with the Arp2/3 complex mediates lamellipodia and actin comet tail formation. J Biol Chem. 281(1):617-25.
· Kohler C. et al., 2004. Expression of Notch-1 and its ligand Jagged-1 in rat liver during liver regeneration. Hepatology. 39(4):1056-65.
· Park CW. et al., 2004. Double-stranded siRNA targeted to the huntingtin gene does not induce DNA methylation. Biochem Biophys Res Commun. 323(1):275-80.
· Phillips N. et al., 2004. Phosphorylation of the enteropathogenic E. coli receptor by the Src-family kinase c-Fyn triggers actin pedestal formation. Nat Cell Biol. 6(7):618-25.
· Stege A. et al., 2004. Stable and complete overcoming of MDR1/P-glycoprotein-mediated multidrug resistance in human gastric carcinoma cells by RNA interference. Cancer Gene Ther. 11(11):699-706.
· Xu XM. et al., 2004. RNA-mediated gene silencing of the RON receptor tyrosine kinase alters oncogenic phenotypes of human colorectal carcinoma cells. Oncogene. 23(52):8464-74.
· Zhang J. et al., 2005. The chemokine stromal cell derived factor-1 (CXCL12) promotes glioma invasiveness through MT2-matrix metalloproteinase. Carcinogenesis. 26(12):2069-77.
psiRNA-h7SK
· Chen K. et al., 2006. Activation of Toll-like Receptor 2 on Microglia Promotes Cell Uptake of Alzheimer Disease-associated Amyloid beta Peptide. J Biol Chem. 281(6):3651-9.
· Mazzanti CM. et al., 2004. Early genetic mechanisms underlying the inhibitory effects of endostatin and fumagillin on human endothelial cells. Genome Res. 14(8):1585-93. [PDF]
· Sullivan CS & Ganem D., 2005. A virus-encoded inhibitor that blocks RNA interference in mammalian cells. J Virol. 79(12):7371-9. [