RAW-Lucia™ ISG-KO-MDA5 Cells

MDA-5 knockout IRF-inducible Lucia luciferase reporter mouse macrophages

RAW-Lucia ISG-KO-MDA5 cells were generated from RAW-Lucia ISG cells through the stable knockout of the MDA-5 gene. These cells derive from the murine RAW 264.7 macrophage cell line, which has been reported to express many pattern recognition receptors (PRRs), including the dsRNA sensors MDA-5 [1] and RIG-I [2,3] along with their adaptor protein IPS-1 (also known as MAVS) [3].

RAW-Lucia ISG-KO-MDA5 and RAW-Lucia ISG cells can be used to study the role of MDA-5 in a mouse macrophage cell line by monitoring of interferon regulatory factor (IRF)-induced Lucia luciferase activity. They express the gene for secreted Lucia luciferase under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE. The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc, a Lucia luciferase detection reagent.

RAW-Lucia ISG-KO-MDA5 cells are resistant to Zeocin.


Antibiotic resistance: Zeocin™

Growth Medium: DMEM, 4.5 g/l glucose, 10% fetal bovine serum (FBS), 100 µg/ml Normocin™, 2 mM L-glutamine

Quality Control: MDA-5 knockout is verified by PCR and DNA sequencing to confirm frameshift mutation/deletion. The cells are guaranteed mycoplasma-free.

This product is covered by a Limited Use License (See Terms and Conditions).


1 vial containing 3-7 x 106 cells

100 μl Zeocin™ (100 mg/ml)

1 ml Normocin™ (50 mg/ml)

1 pouch of QUANTI-Luc™ (Luciferase detection medium)

Shipped on dry ice


1. Hasan M. et al., 2011. Antimicrobial peptides inhibit polyinosinic-polycytidylic acid-induced immune responses. J Immunol. 187(11):5653-9.
2. Melchjorsen J. et al., 2005. Activation of innate defense against a paramyxovirus is mediated by RIG-I and TLR7 and TLR8 in a cell-type-specific manner. J Virol. 79:12944-51.
3. Yamashita M. et al., 2013. Antiviral innate immunity disturbs podocyte cell function. J Innate Immun. 5:231-41.


RAW-Lucia™ ISG-KO-MDA5 Cells

Description MDA-5 Knockout IRF-Lucia Luciferase Reporter Cell Line
Cat. Coderawl-komda5
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
please select your country
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
Copyrights © 2011-2016 InvivoGen. All Rights Reserved. Reproduction of any materials from this site is strictly forbidden without permission for commercial use. Nonprofit use for non-commercial research and educational purposes is permitted, citation should include the URL "www.invivogen.com".