psiRNA-h7SK

7SK-based expression of shRNAs

psiRNA-h7SK is a family of expression vectors designed to generate shRNAs from the human 7SK RNA polymerase III promoter. 7SK is an abundant and evolutionarily conserved small nuclear RNA transcribed by RNA polymerase III [1]. The human 7SK promoter is ideal for the production of shRNAs as it can generate high amounts of shRNAs [1, 2]. It has been shown to provide better silencing efficiencies of various target genes than the human H1 and U6 promoters. Furthermore, the human 7SK promoter can be used in combination with other RNA pol III promoters for multiple shRNA expression strategies [6]. psiRNA-h7SK has been successfully used to achieve gene silencing in vitro and in vivo [3-5].

Specifications

Two cloning options: Acc 65I / Hind III or Bbs I / Bbs I

Allows white and blue selection

Available with different selectable markers: blasticidin, hygromycin, kanamycin/G418, Zeocin™, GFP::Zeo

Provided with the psiRNA™ tools:
• 1 disk of lyophilized GT116 E. coli bacteria
• Sequencing primers (OL559-OL408)
E. coli Fast-Media® XGal

Contents

Each psiRNA-h7SK plasmid is provided with the following components:

- 20 μg of the psiRNA-h7SK plasmid of your choice (see list)
- 20 μg of control plasmid 1 (targets the EGFP or LacZ reporter genes)
- 20 μg of control plasmid 2 (targets the luciferase GL3 reporter gene)
- 1 disk of lyophilized GT116 E. coli bacteria (GT116 genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZM15
ΔlacX74 recA1 endA1 ΔsbcC-sbcD)
- 10 μg of OL559 primer (forward) and 10 μg of OL408 primer (reverse)
- 4 pouches of corresponding Fast-Media® XGal

Details

psiRNA-h7SK backbone

Ref.

1. Czauderna F. et al., 2003. Inducible shRNA expression for application in a prostate cancer mouse model. Nucleic Acids Res. 31(21):e127.

2. Koper-Emde D. et al., 2004. RNA interference by small hairpin RNAs synthesised under control of the human 7S K RNA promoter. Biol Chem. 385(9):791-4.

3. ter Brake O. et al., 2008. Lentiviral Vector Design for Multiple shRNA Expression and Durable HIV-1 Inhibition. Mol Ther. 16(3):557-64.

4. Triantafilou K. et al., 2005. Human cardiac inflammatory responses triggered by Coxsackie B viruses are mainly Toll-like receptor (TLR) 8-dependent. Cell Microbiol. 7(8):1117-26.

5. Tajeddine N. et al., 2005. Tumor-associated antigen preferentially expressed antigen of melanoma (PRAME) induces caspase-independent cell death in vitro and reduces tumorigenicity in vivo. Cancer Res.65(16):7348-55.

6. Mazzanti CM. et al., 2004. Early genetic mechanisms underlying the inhibitory effects of endostatin and fumagillin on human endothelial cells. Genome Res. 14(8):1585-93.

 

Citations

Recent articles using InvivoGen psiRNA-h7SK

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psiRNA-h7SK G1 GFPzeo

Cat. Codeksirna4-gz21
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psiRNA-h7SK G1 Blasti

Cat. Codeksirna3-b21
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psiRNA-h7SK G1 Hygro

Cat. Codeksirna3-h21
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psiRNA-h7SK G1 Neo

Cat. Codeksirna3-n21
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psiRNA-h7SK G1 Zeo

Cat. Codeksirna3-z21
Unit Size1 kit
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