psiRNA-h7SK
7SK-based expression of shRNAs
psiRNA-h7SK is a family of expression vectors designed to generate shRNAs from the human 7SK RNA polymerase III promoter. 7SK is an abundant and evolutionarily conserved small nuclear RNA transcribed by RNA polymerase III [1]. The human 7SK promoter is ideal for the production of shRNAs as it can generate high amounts of shRNAs [1, 2]. It has been shown to provide better silencing efficiencies of various target genes than the human H1 and U6 promoters. Furthermore, the human 7SK promoter can be used in combination with other RNA pol III promoters for multiple shRNA expression strategies [6]. psiRNA-h7SK has been successfully used to achieve gene silencing in vitro and in vivo [3-5].
Specifications
Two cloning options: Acc 65I / Hind III or Bbs I / Bbs I
Allows white and blue selection
Available with different selectable markers: blasticidin, hygromycin, kanamycin/G418, Zeocin™, GFP::Zeo
Provided with the psiRNA™ tools:
• LyoComp GT116, lyophilized chemically competent E. coli GT116 cells
• Sequencing primers (OL559-OL408)
• E. coli Fast-Media® XGal
Documents
- TDS psiRNA-h7SK G1 Blasti : 1 kit (ksirna3-b21)
- TDS psiRNA-h7SK G1 Hygro : 1 kit (ksirna3-h21)
- TDS psiRNA-h7SK G1 Neo : 1 kit (ksirna3-n21)
- TDS psiRNA-h7SK G1 Zeo : 1 kit (ksirna3-z21)
- TDS psiRNA-h7SK G1 GFPzeo : 1 kit (ksirna4-gz21)
- Sequence psiRNA-h7SK G1 Blasti : 1 kit (ksirna3-b21)
- Sequence psiRNA-h7SK G1 Hygro : 1 kit (ksirna3-h21)
- Sequence psiRNA-h7SK G1 Neo : 1 kit (ksirna3-n21)
- Sequence psiRNA-h7SK G1 Zeo : 1 kit (ksirna3-z21)
- Sequence psiRNA-h7SK G1 GFPzeo : 1 kit (ksirna4-gz21)
Contents
Each psiRNA-h7SK plasmid is provided with the following components:
- 20 μg of the psiRNA-h7SK plasmid of your choice (see list)
- 20 μg of control plasmid 1 (targets the EGFP or LacZ reporter genes)
- 20 μg of control plasmid 2 (targets the luciferase GL3 reporter gene)
- 1 vial of LyoComp GT116 with 2 ml Reconstitutive Solution
- 10 μg of OL559 primer (forward) and 10 μg of OL408 primer (reverse)
- 4 pouches of corresponding Fast-Media® XGal
Details
Ref.
1. Czauderna F. et al., 2003. Inducible shRNA expression for application in a prostate cancer mouse model. Nucleic Acids Res. 31(21):e127.
2. Koper-Emde D. et al., 2004. RNA interference by small hairpin RNAs synthesised under control of the human 7S K RNA promoter. Biol Chem. 385(9):791-4.
3. ter Brake O. et al., 2008. Lentiviral Vector Design for Multiple shRNA Expression and Durable HIV-1 Inhibition. Mol Ther. 16(3):557-64.
4. Triantafilou K. et al., 2005. Human cardiac inflammatory responses triggered by Coxsackie B viruses are mainly Toll-like receptor (TLR) 8-dependent. Cell Microbiol. 7(8):1117-26.
5. Tajeddine N. et al., 2005. Tumor-associated antigen preferentially expressed antigen of melanoma (PRAME) induces caspase-independent cell death in vitro and reduces tumorigenicity in vivo. Cancer Res.65(16):7348-55.
6. Mazzanti CM. et al., 2004. Early genetic mechanisms underlying the inhibitory effects of endostatin and fumagillin on human endothelial cells. Genome Res. 14(8):1585-93.
ORDERING
psiRNA-h7SK G1 Blasti
| Cat. Code | ksirna3-b21 |
|---|---|
| Unit Size | 1 kit |
| Price | For price or distributor address, please select your country |
psiRNA-h7SK G1 Hygro
| Cat. Code | ksirna3-h21 |
|---|---|
| Unit Size | 1 kit |
| Price | For price or distributor address, please select your country |
psiRNA-h7SK G1 Neo
| Cat. Code | ksirna3-n21 |
|---|---|
| Unit Size | 1 kit |
| Price | For price or distributor address, please select your country |
psiRNA-h7SK G1 Zeo
| Cat. Code | ksirna3-z21 |
|---|---|
| Unit Size | 1 kit |
| Price | For price or distributor address, please select your country |
psiRNA-h7SK G1 GFPzeo
| Cat. Code | ksirna4-gz21 |
|---|---|
| Unit Size | 1 kit |
| Price | For price or distributor address, please select your country |
