pNiFty2-IFB plasmid is composed of two key elements:  the mouse interferon beta minimal promoter and a  SEAP (Secreted alkaline phosphatase) reporter gene.

pNiFty2-IFB plasmid is selectable with Zeocin™ in both E. coli and mammalian cells, and can be used to generate stable clones.


Minimal Promoter: mouse IFNβ promoter
Selection: Zeocin™
Reporter Gene: SEAP


pNiFty2-IFB plasmid is provided as 20 µg lyophilized DNA with 4 pouches of Fast-Media® (2 TB and 2 Agar), containing Zeocin™.

Products are shipped at room temperature and should be stored at -20°C.


Minimal promoter

The proximal promoters are shorter than 500 bp and contain transcription factor binding sites. Upon stimulation in 293 cells, their expression level remains undetectable. With the addition of repeated TFBS, the proximal promoters become inducible by the appropriate stimulus and drive the expression of the reporter gene.

mIFNβ promoter: The mouse interferon beta minimal promoter comprises several positive regulatory domains (PRDs) that bind different cooperating transcription factors such as NF-κB, IRF3 and IRF7 [1]. Co-expression of IFNβ-SEAP with constitutively activated IRF3 (saIRF3) or IRF7 (saIRF7) in HEK293 cells led to a strong increase in SEAP expression.

Reporter Gene

SEAP reporter gene: Secreted alkaline phosphatase (SEAP) is a reporter widely used to study promoter activity or gene expression. SEAP expression can be rapidly and readily measured in supernatants of transfected cells. SEAP levels can be evaluated qualitatively with the naked eye and quantitatively using SEAP detection media, such as HEK-Blue™ Detection system or the SEAP Reporter Assay.

1. Vodjdani G. et al. 1998. Structure and characterization of a murine chromosomal fragment containing the interferon beta gene. J Mol Biol. 204(2):221-31.



Description pNiFty2 : mIFNβ - ZeoR - SEAP
Cat. Codepnf2-ifbsp
Unit Size20 µg
Price For price or distributor address,
please select your country
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
Copyrights © 2011-2016 InvivoGen. All Rights Reserved. Reproduction of any materials from this site is strictly forbidden without permission for commercial use. Nonprofit use for non-commercial research and educational purposes is permitted, citation should include the URL "".