Invivogen
Menu

pFUSE2-CLIg-ratL1

Product Unit size Cat. code Docs. Qty. Price

pFUSE2-CLIg-ratL1

Rat lambda 1

Show product

20 µg

pfuse2-rtcll1
+-
$684

pFUSE2ss-CLIg-ratL1

Rat lambda 1

Show product

20 µg

pfuse2ss-rtcll1
+-
$684

Light chain expression plasmid - Rat lambda 1

pFUSE2-CLIg-ratL1 is a cloning plasmid that expresses the constant region of the rat lambda 1 light chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the light chain variable region.

This plasmid is designed for antibody generation when co-transfected into mammalian cells with the heavy chain variable region cloned into pFUSE-CHIg.

pFUSE2ss-CLIg-ratL1 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

Back to the top

Specifications

  • pFUSE2-CLIg-ratL1 features the constant region of the mouse Ig lambda 1 light chain
  •  pFUSE2ss-CLIg-ratL1 features the constant region of the mouse Ig lambda 1 light chain and the IL2 signal sequence

Both plasmids are selectable with blasticidin.

Back to the top

pFUSE2 Contents

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

Alert Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.

 

Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.

Avoid repeated freeze-thaw cycles.

Back to the top
Customer Service
& Technical Support
Shopping cart is empty