pFUSE-mIgG2Ae1-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the murine IgG2 heavy chain mutated L235E + E318A/K320A/K322A and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently.

mIgG2Ae1-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.


Isotype : IgG2a mouse
: L235E + E318A/K320A/K322A

- Reduced binding to FcγRI and C1q
- Reduced ADCC and CDC

Both plasmids are selectable with Zeocin™.


• 20 μg of lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)


Steurer W. et al., 1995. Ex vivo coating of islet cell allografts with murine CTLA4/Fc promotes graft tolerance. J Immunol. 155(3):1165-74.



Cat. Codepfc1-mg2ae1
Unit Size20 µg
Price For price or distributor address,
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Cat. Codepfc2-mg2ae1
Unit Size20 µg
Price For price or distributor address,
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